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A Butyl Methacrylate Monolithic Column Prepared In-Situ on a Microfluidic Chip and its Applications
National Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, and International R&D Center of Micro-Nano Systems and New Materials Technology, and Microsystem Research Center, and College of Chemistry and Chemical Engineering, China
* Author to whom correspondence should be addressed.
Received: 6 February 2009; in revised form: 3 March 2009 / Accepted: 21 April 2009 / Published: 8 May 2009
Abstract: A butyl methacrylate (BMA) monolithic column was polymerized in-situ with UV irradiation in an ultraviolet transparent PDMS micro-channel on a homemade micro-fluidic chip. Under the optimized conditions and using a typical polymerization mixture consisting of 75% porogenic solvents and 25% monomers, the BMA monolithic column was obtained as expected. The BET surface area ratio of the BMA monolithic column was 366 m2·g-1. The corresponding SEM images showed that the monolithic column material polymerized in a glass channel was composed of uniform pores and spherical particles with diameters ranging from 3 to 5 μm. The promethazine–luminal–potassium ferricyanide chemiluminescence system was selected for testing the capability of the column. A flow injection analytical technique–chemiluminescence (FIA–CL) system on the microfluidic chip with a BMA monolithic column pretreatment unit was established to determine promethazine. Trace promethazine was enriched by the BMA monolithic column, with more than a 10-fold average enrichment ratio. The proposed method has a linear response concentration range of 1.0×10-8 - 1.0×10-6g·mL-1 and the detection limit was 1.6×10-9g·mL-1.
Keywords: BMA monolithic column; separation and preconcentration; microfluidic chip
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MDPI and ACS Style
Xu, Y.; Zhang, W.; Zeng, P.; Cao, Q. A Butyl Methacrylate Monolithic Column Prepared In-Situ on a Microfluidic Chip and its Applications. Sensors 2009, 9, 3437-3446.
Xu Y, Zhang W, Zeng P, Cao Q. A Butyl Methacrylate Monolithic Column Prepared In-Situ on a Microfluidic Chip and its Applications. Sensors. 2009; 9(5):3437-3446.
Xu, Yi; Zhang, Wenpin; Zeng, Ping; Cao, Qiang. 2009. "A Butyl Methacrylate Monolithic Column Prepared In-Situ on a Microfluidic Chip and its Applications." Sensors 9, no. 5: 3437-3446.