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Detection of Biomolecular Binding Through Enhancement of Localized Surface Plasmon Resonance (LSPR) by Gold Nanoparticles
Fusion Biotechnology Research Center, KRICT, Daejeon 305-600, Republic of Korea
BioMonitoring Research Center, Korea Research Institute of Bioscience and Biotechnology, Yusong-gu, Daejeon, 305–333, Korea
* Author to whom correspondence should be addressed.
Received: 13 March 2009; in revised form: 23 March 2009 / Accepted: 30 March 2009 / Published: 30 March 2009
Abstract: To amplify the difference in localized surface plasmon resonance (LSPR) spectra of gold nano-islands due to intermolecular binding events, gold nanoparticles were used. LSPR-based optical biosensors consisting of gold nano-islands were readily made on glass substrates using evaporation and heat treatment. Streptavidin (STA) and biotinylated bovine serum albumin (Bio-BSA) were chosen as the model receptor and the model analyte, respectively, to demonstrate the effectiveness of this detection method. Using this model system, we were able to enhance the sensitivity in monitoring the binding of Bio-BSA to gold nano-island surfaces functionalized with STA through the addition of gold nanoparticle-STA conjugates. In addition, SU-8 well chips with gold nano-island surfaces were fabricated through a conventional UV patterning method and were then utilized for image detection using the attenuated total reflection mode. These results suggest that the gold nano-island well chip may have the potential to be used for multiple and simultaneous detection of various bio-substances.
Keywords: Localized surface plasmon resonance (LSPR); gold nano-island; gold nanoparticle; attenuated total reflection (ATR); well chip
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Cite This Article
MDPI and ACS Style
Kim, H.M.; Jin, S.M.; Lee, S.K.; Kim, M.-G.; Shin, Y.-B. Detection of Biomolecular Binding Through Enhancement of Localized Surface Plasmon Resonance (LSPR) by Gold Nanoparticles. Sensors 2009, 9, 2334-2344.
Kim HM, Jin SM, Lee SK, Kim M-G, Shin Y-B. Detection of Biomolecular Binding Through Enhancement of Localized Surface Plasmon Resonance (LSPR) by Gold Nanoparticles. Sensors. 2009; 9(4):2334-2344.
Kim, Hyung Min; Jin, Seung Min; Lee, Seok Kee; Kim, Min-Gon; Shin, Yong-Beom. 2009. "Detection of Biomolecular Binding Through Enhancement of Localized Surface Plasmon Resonance (LSPR) by Gold Nanoparticles." Sensors 9, no. 4: 2334-2344.