Micro-Pattern Guided Adhesion of Osteoblasts on Diamond Surfaces
AbstractMicroscopic chemical patterning of diamond surfaces by hydrogen and oxygen surface atoms is used for self-assembly of human osteoblastic cells into micro-arrays. The cell adhesion and assembly is further controlled by concentration of cells (2,500-10,000 cells/cm2)and fetal bovine serum (0-15%). The cells are characterized by fluorescence microscopy of actin fibers and nuclei. The serum protein adsorption is studied by atomic force microscopy (AFM). The cells are arranged selectively on O-terminated patterns into 30-200 μm wide arrays. Higher cell concentrations allow colonization of unfavorable H-terminated regions due to mutual cell communication. There is no cell selectivity without the proteins in the medium. Based on the AFM, the proteins are present on both H- and O-terminated surfaces. Pronounced differences in their thickness, surface roughness, morphology, and phase imagesindicate different conformation of the proteins and explain the cell selectivity.
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Rezek, B.; Michalíková, L.; Ukraintsev, E.; Kromka, A.; Kalbacova, M. Micro-Pattern Guided Adhesion of Osteoblasts on Diamond Surfaces. Sensors 2009, 9, 3549-3562.
Rezek B, Michalíková L, Ukraintsev E, Kromka A, Kalbacova M. Micro-Pattern Guided Adhesion of Osteoblasts on Diamond Surfaces. Sensors. 2009; 9(5):3549-3562.Chicago/Turabian Style
Rezek, Bohuslav; Michalíková, Lenka; Ukraintsev, Egor; Kromka, Alexander; Kalbacova, Marie. 2009. "Micro-Pattern Guided Adhesion of Osteoblasts on Diamond Surfaces." Sensors 9, no. 5: 3549-3562.