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Micro-Pattern Guided Adhesion of Osteoblasts on Diamond Surfaces
Institute of Physics, Academy of Sciences of the Czech Republic, Cukrovarnicka 10, Prague 6, Czech Republic
Faculty of Electrical Engineering and Information Technology, Slovak University of Technology, Ilkovičova 3, Bratislava, Slovak Republic
Institute of Inherited Metabolic Disorders, 1st Faculty of Medicine, Charles University, Ke Karlovu 2, Prague 2, Czech Republic
* Authors to whom correspondence should be addressed.
Received: 10 March 2009; in revised form: 7 April 2009 / Accepted: 5 May 2009 / Published: 13 May 2009
Abstract: Microscopic chemical patterning of diamond surfaces by hydrogen and oxygen surface atoms is used for self-assembly of human osteoblastic cells into micro-arrays. The cell adhesion and assembly is further controlled by concentration of cells (2,500-10,000 cells/cm2)and fetal bovine serum (0-15%). The cells are characterized by fluorescence microscopy of actin fibers and nuclei. The serum protein adsorption is studied by atomic force microscopy (AFM). The cells are arranged selectively on O-terminated patterns into 30-200 μm wide arrays. Higher cell concentrations allow colonization of unfavorable H-terminated regions due to mutual cell communication. There is no cell selectivity without the proteins in the medium. Based on the AFM, the proteins are present on both H- and O-terminated surfaces. Pronounced differences in their thickness, surface roughness, morphology, and phase imagesindicate different conformation of the proteins and explain the cell selectivity.
Keywords: cell adhesion; proteins; diamond; atomic force microscopy; biotechnology; tissue engineering; biosensors; osteoblasts
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Rezek, B.; Michalíková, L.; Ukraintsev, E.; Kromka, A.; Kalbacova, M. Micro-Pattern Guided Adhesion of Osteoblasts on Diamond Surfaces. Sensors 2009, 9, 3549-3562.
Rezek B, Michalíková L, Ukraintsev E, Kromka A, Kalbacova M. Micro-Pattern Guided Adhesion of Osteoblasts on Diamond Surfaces. Sensors. 2009; 9(5):3549-3562.
Rezek, Bohuslav; Michalíková, Lenka; Ukraintsev, Egor; Kromka, Alexander; Kalbacova, Marie. 2009. "Micro-Pattern Guided Adhesion of Osteoblasts on Diamond Surfaces." Sensors 9, no. 5: 3549-3562.