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Sensors 2009, 9(5), 3549-3562; doi:10.3390/s90503549

Micro-Pattern Guided Adhesion of Osteoblasts on Diamond Surfaces

1,* , 1, 2,* , 1
1 Institute of Physics, Academy of Sciences of the Czech Republic, Cukrovarnicka 10, Prague 6, Czech Republic 2 Faculty of Electrical Engineering and Information Technology, Slovak University of Technology, Ilkovičova 3, Bratislava, Slovak Republic 3 Institute of Inherited Metabolic Disorders, 1st Faculty of Medicine, Charles University, Ke Karlovu 2, Prague 2, Czech Republic
* Authors to whom correspondence should be addressed.
Received: 10 March 2009 / Revised: 7 April 2009 / Accepted: 5 May 2009 / Published: 13 May 2009
(This article belongs to the Special Issue Nanotechnological Advances in Biosensors)
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Microscopic chemical patterning of diamond surfaces by hydrogen and oxygen surface atoms is used for self-assembly of human osteoblastic cells into micro-arrays. The cell adhesion and assembly is further controlled by concentration of cells (2,500-10,000 cells/cm2)and fetal bovine serum (0-15%). The cells are characterized by fluorescence microscopy of actin fibers and nuclei. The serum protein adsorption is studied by atomic force microscopy (AFM). The cells are arranged selectively on O-terminated patterns into 30-200 μm wide arrays. Higher cell concentrations allow colonization of unfavorable H-terminated regions due to mutual cell communication. There is no cell selectivity without the proteins in the medium. Based on the AFM, the proteins are present on both H- and O-terminated surfaces. Pronounced differences in their thickness, surface roughness, morphology, and phase imagesindicate different conformation of the proteins and explain the cell selectivity.
Keywords: cell adhesion; proteins; diamond; atomic force microscopy; biotechnology; tissue engineering; biosensors; osteoblasts cell adhesion; proteins; diamond; atomic force microscopy; biotechnology; tissue engineering; biosensors; osteoblasts
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Rezek, B.; Michalíková, L.; Ukraintsev, E.; Kromka, A.; Kalbacova, M. Micro-Pattern Guided Adhesion of Osteoblasts on Diamond Surfaces. Sensors 2009, 9, 3549-3562.

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