Next Article in Journal
Segment Tracking via a Spatiotemporal Linking Process including Feedback Stabilization in an n-D Lattice Model
Next Article in Special Issue
Use of Biosensors as Alternatives to Current Regulatory Methods for Marine Biotoxins
Previous Article in Journal
The Development of a Portable Hard Disk Encryption/Decryption System with a MEMS Coded Lock
Previous Article in Special Issue
Sensing Mercury for Biomedical and Environmental Monitoring
Sensors 2009, 9(11), 9332-9354; doi:10.3390/s91109332
Article

Synthesis and Characterization of Anti-HER2 Antibody Conjugated CdSe/CdZnS Quantum Dots for Fluorescence Imaging of Breast Cancer Cells

1
,
2
,
2
,
1
,
1
 and
1,2,*
1 WPI Immunology Frontier Research Center, Osaka University, Yamada-oka 1-3, Suita, Osaka 565-0871, Japan 2 Graduate School of Frontier Biosciences, Osaka University, Yamada-oka 2-1, Suita, Osaka 565-0871, Japan
* Author to whom correspondence should be addressed.
Received: 21 September 2009 / Revised: 3 November 2009 / Accepted: 12 November 2009 / Published: 19 November 2009
(This article belongs to the Special Issue Fluorescent Chemosensors)
View Full-Text   |   Download PDF [3226 KB, uploaded 21 June 2014]   |   Browse Figures

Abstract

The early detection of HER2 (human epidermal growth factor receptor 2) status in breast cancer patients is very important for the effective implementation of anti-HER2 antibody therapy. Recently, HER2 detections using antibody conjugated quantum dots (QDs) have attracted much attention. QDs are a new class of fluorescent materials that have superior properties such as high brightness, high resistance to photo-bleaching, and multi-colored emission by a single-light source excitation. In this study, we synthesized three types of anti-HER2 antibody conjugated QDs (HER2Ab-QDs) using different coupling agents (EDC/sulfo-NHS, iminothiolane/sulfo-SMCC, and sulfo-SMCC). As water-soluble QDs for the conjugation of antibody, we used glutathione coated CdSe/CdZnS QDs (GSH-QDs) with fluorescence quantum yields of 0.23~0.39 in aqueous solution. Dispersibility, hydrodynamic size, and apparent molecular weights of the GSH-QDs and HER2Ab-QDs were characterized by using dynamic light scattering, fluorescence correlation spectroscopy, atomic force microscope, and size-exclusion HPLC. Fluorescence imaging of HER2 overexpressing cells (KPL-4 human breast cancer cell line) was performed by using HER2Ab-QDs as fluorescent probes. We found that the HER2Ab-QD prepared by using SMCC coupling with partially reduced antibody is a most effective probe for the detection of HER2 expression in KPL-4 cells. We have also studied the size dependency of HER2Ab-QDs (with green, orange, and red emission) on the fluorescence image of KPL-4 cells.
Keywords: quantum dots; HER2; antibody; cellular imaging; breast cancer cell quantum dots; HER2; antibody; cellular imaging; breast cancer cell
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
SciFeed

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
RIS
MDPI and ACS Style

Tiwari, D.K.; Tanaka, S.-I.; Inouye, Y.; Yoshizawa, K.; Watanabe, T.M.; Jin, T. Synthesis and Characterization of Anti-HER2 Antibody Conjugated CdSe/CdZnS Quantum Dots for Fluorescence Imaging of Breast Cancer Cells. Sensors 2009, 9, 9332-9354.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert