Next Article in Journal
An Immunity-Based Anomaly Detection System with Sensor Agents
Next Article in Special Issue
Circular High-Q Resonating Isotropic Strain Sensors with Large Shift of Resonance Frequency under Stress
Previous Article in Journal
Measuring Gait Using a Ground Laser Range Sensor
Sensors 2009, 9(11), 9147-9174; doi:10.3390/s91109147
Review

Reporter Proteins in Whole-Cell Optical Bioreporter Detection Systems, Biosensor Integrations, and Biosensing Applications

,
 and
*
Received: 18 September 2009 / Revised: 14 October 2009 / Accepted: 23 October 2009 / Published: 17 November 2009
(This article belongs to the Special Issue Microbial Sensors and Biosensors)
View Full-Text   |   Download PDF [607 KB, uploaded 21 June 2014]   |   Browse Figures

Abstract

Whole-cell, genetically modified bioreporters are designed to emit detectable signals in response to a target analyte or related group of analytes. When integrated with a transducer capable of measuring those signals, a biosensor results that acts as a self-contained analytical system useful in basic and applied environmental, medical, pharmacological, and agricultural sciences. Historically, these devices have focused on signaling proteins such as green fluorescent protein, aequorin, firefly luciferase, and/or bacterial luciferase. The biochemistry and genetic development of these sensor systems as well as the advantages, challenges, and common applications of each one will be discussed.
Keywords: aequorin; bacterial luciferase (Lux); bioreporter; biosensor; firefly luciferase (Luc); green fluorescent protein (GFP) aequorin; bacterial luciferase (Lux); bioreporter; biosensor; firefly luciferase (Luc); green fluorescent protein (GFP)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Close, D.M.; Ripp, S.; Sayler, G.S. Reporter Proteins in Whole-Cell Optical Bioreporter Detection Systems, Biosensor Integrations, and Biosensing Applications. Sensors 2009, 9, 9147-9174.

View more citation formats

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert