Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma

doi:10.3390/s91008271

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Sensors 2009, 9(10), 8271-8277; doi:10.3390/s91008271

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Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma

Takehito Ogawa¹, Satoka Aoyagi², Takehiro Miyasaka³ and Kiyotaka Sakai^1,*

¹ Department of Chemical Engineering, Waseda University / 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan ² Faculty of Life and Environmental Science, Shimane University / 1060 Nishikawatsu-cho, Matsue-shi, Shimane 690-8504, Japan ³ Department of Medical Engineering, Himeji Dokkyo University / 7-2-1 Kamiono, Himeji-shi, Hyogo 670-8524, Japan

* Author to whom correspondence should be addressed.

Received: 27 August 2009; in revised form: 29 September 2009 / Accepted: 15 October 2009 / Published: 20 October 2009

(This article belongs to the Section Biosensors)

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Abstract: Fluorescence enhancement of fluorescein isothiocyanate-labeled protein A (FITC-protein A) caused by the binding with immunoglobulin G (IgG) in bovine plasma was studied. FITC-protein A was immobilized onto a glass surface by covalent bonds. An increase in fluorescence intensity was dependent on IgG concentration ranging from 20 to 78 μg/mL in both phosphate buffer saline and bovine plasma. This method requires no separation procedure, and the reaction time is less than 15 min. A fluorescence enhancement assay by the affinity binding of fluorescence-labeled reagent is thus available for the rapid determination of biomolecules in plasma.

Keywords: fluorescence enhancement; immunoassay; immunoglobulin G; Protein A

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MDPI and ACS Style

Ogawa, T.; Aoyagi, S.; Miyasaka, T.; Sakai, K. Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma. Sensors 2009, 9, 8271-8277.

AMA Style

Ogawa T., Aoyagi S., Miyasaka T., Sakai K. Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma. Sensors. 2009; 9(10):8271-8277.

Chicago/Turabian Style

Ogawa, Takehito; Aoyagi, Satoka; Miyasaka, Takehiro; Sakai, Kiyotaka. 2009. "Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma." Sensors 9, no. 10: 8271-8277.

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