Next Article in Journal
Cross-Layer Active Predictive Congestion Control Protocol for Wireless Sensor Networks
Previous Article in Journal
Major Improvements of Quartz Crystal Pulling Sensitivity and Linearity Using Series Reactance
Article Menu

Export Article

Open AccessCommunication
Sensors 2009, 9(10), 8271-8277; doi:10.3390/s91008271

Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma

1
Department of Chemical Engineering, Waseda University / 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan
2
Faculty of Life and Environmental Science, Shimane University / 1060 Nishikawatsu-cho, Matsue-shi, Shimane 690-8504, Japan
3
Department of Medical Engineering, Himeji Dokkyo University / 7-2-1 Kamiono, Himeji-shi, Hyogo 670-8524, Japan
*
Author to whom correspondence should be addressed.
Received: 27 August 2009 / Revised: 29 September 2009 / Accepted: 15 October 2009 / Published: 20 October 2009
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [198 KB, uploaded 21 June 2014]   |  

Abstract

Fluorescence enhancement of fluorescein isothiocyanate-labeled protein A (FITC-protein A) caused by the binding with immunoglobulin G (IgG) in bovine plasma was studied. FITC-protein A was immobilized onto a glass surface by covalent bonds. An increase in fluorescence intensity was dependent on IgG concentration ranging from 20 to 78 μg/mL in both phosphate buffer saline and bovine plasma. This method requires no separation procedure, and the reaction time is less than 15 min. A fluorescence enhancement assay by the affinity binding of fluorescence-labeled reagent is thus available for the rapid determination of biomolecules in plasma. View Full-Text
Keywords: fluorescence enhancement; immunoassay; immunoglobulin G; Protein A fluorescence enhancement; immunoassay; immunoglobulin G; Protein A
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Ogawa, T.; Aoyagi, S.; Miyasaka, T.; Sakai, K. Fluorescence Enhancement of Fluorescein Isothiocyanate-Labeled Protein A Caused by Affinity Binding with Immunoglobulin G in Bovine Plasma. Sensors 2009, 9, 8271-8277.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top