- freely available
Sensors 2009, 9(10), 8130-8145; doi:10.3390/s91008130
Published: 16 October 2009
Abstract: Many types of biosensors employ magnetic nanoparticles (diameter = 5–300 nm) or magnetic particles (diameter = 300–5,000 nm) which have been surface functionalized to recognize specific molecular targets. Here we cover three types of biosensors that employ different biosensing principles, magnetic materials, and instrumentation. The first type consists of magnetic relaxation switch assay-sensors, which are based on the effects magnetic particles exert on water proton relaxation rates. The second type consists of magnetic particle relaxation sensors, which determine the relaxation of the magnetic moment within the magnetic particle. The third type is magnetoresistive sensors, which detect the presence of magnetic particles on the surface of electronic devices that are sensitive to changes in magnetic fields on their surface. Recent improvements in the design of magnetic nanoparticles (and magnetic particles), together with improvements in instrumentation, suggest that magnetic material-based biosensors may become widely used in the future.
Nanoscale magnetic materials are an important source of labels for biosensing due to their strong magnetic properties which are not found in biological systems. Modulation of the composition, size and magnetic properties of these materials permits their use in a variety of instruments and formats for biosensing [1,2]. New types of instrumentation are promising for the use of nanoscale magnetic materials in point of care sensors in variety of applications. Here, we cover three biosensors that employ magnetic nanoparticle labels with different sensing principles and instrumentation: (i) magnetic relaxation switches, (ii) magnetic particle relaxation sensors, and (iii) magnetoresistive sensors.
2. Magnetic Relaxation Switches (MRSws)
Superparamagnetic nanoparticles made of iron oxide and a polymeric coating are clinically proven magnetic resonance (MR) contrast agents and widely used in pre-clinical, targeted molecular imaging applications [2,3]. When used as targeted contrast agents, surface-modified nanoparticles (NPs) bind specific molecules producing local inhomogenieties in the applied magnetic field in tissues where molecular targets are present. These inhomogeneities result in decreases in the T2 relaxation time (or increases in 1/T2, the T2 relaxation rate), and these, in turn, lead to changes in the contrast of MR images.
Recently, Josephson and collaborators exploited the change in T2 produced by magnetic NPs to obtain MR based assays called Magnetic Relaxation Switches (MRSws). The principle of MRSw assays is illustrated in Figure 1; NPs switch between dispersed and aggregated states, and associated with the change in aggregation are changes in the spin-spin relaxation time (T2), the basis of which is discussed below . The materials used in MRSw assays are either magnetic nanoparticles (NPs, diameter 5–300 nm) or micometer-sized magnetic particles (MPs, diameter 300–5,000 nm). As shown in Figure 1, MRSws are homogeneous particle aggregation/disaggregation-based assays similar to aggregation assays using Latex particles, red blood cell hemagglutination, and antibody reactions with proteins (nephelometry). Unlike optically-based assays, MRSws employ radiofrequency radiation which penetrates biological samples regardless of their optical properties . Since the dispersed and aggregated states of NPs (or MPs) can be reversed by such factors such as temperature, pH, and a high concentration of competing analytes, and hence are referred to as “relaxation switches”. The aggregated and dispersed states of magnetic NPs or MPs have different transverse spin-spin relaxation times (values of T2). NP aggregation and the size range of the resulting aggregates depends on the type of analyte and analyte concentration .
2.1. Mechanism of MRSws
MRSw assays exploit the fact that for both nanoparticles (NPs) and larger magnetic particles (MPs) transverse relaxation times (T2) differ between dispersed and aggregated states. However, for Type I, NP-based systems, T2 decreases with the aggregation, while with type II, MP-based systems T2 increases with aggregation. The basis of this is as follows [6–8].
The general theory of how magnetic spheres alter T2 is termed outer sphere relaxation theory. This theory uses two parameters of Dw and tD. Dw is the difference in angular frequencies between the local field experienced by a proton at the equatorial line of the sphere's surface and in the bulk (Dw = mOMg/3, where mO is the vacuum magnetic permeability, M is the particle magnetization, and g is the proton gyromagnetic ratio). Then tD is the translational diffusion time of water around the sphere (tD = Ra2/D, where Ra is the sphere radius and D is the water diffusion coefficient). The outer sphere diffusion theory is applied when the motional average condition is fulfilled as DwtD < 1 [7,8]. In this condition, the relaxation rate R2 (= 1/T2) increases as the sphere's size is increased. As the definitions of Dw and tD imply, the motional average condition is not fulfilled with increased size of the particles such as MPs (DwtD > 1) and the relaxation rate of 1/T2 decreases with the formation of MP aggregates. See the detailed discussion of this phenomenon in a review .
Thus, when present in solution magnetic NPs (or MPs) induce local magnetic field inhomogeneities, which cause a dephasing (loss of phase coherence) of the proton spin precession, and these inhomgeoneities lead to a reduction of the T2 relaxation time. When NPs aggregate (Type I MRSw), a smaller number of larger magnetic field inhomogeneities result. These larger inhomogeneities are more effective dephasers of proton relaxation and T2 drops. Here DwtD < 1. When MPs aggregate (Type II MRSw), a smaller number of larger magnetic field inhomogeneities again results. However, there now so few aggregates, and spaces between them so great, that many water proteins fail to diffuse in and out of these homogeneities during the time course of the measurement. This is termed the “diffusion limited case” for the enhancement of proton relaxation by magnetic microspheres. Here DwtD > 1.
Relaxivity is an important measure of the potency of magnetic materials and an important factor to selecting evaluating materials for use in MRSw assays. Materials with higher relaxivities are more detectable by the relaxometry and can detect lower concentrations of analyte .where R2 is relaxivity of the particle (in moles of metal) expressed as (mM sec)−1, C is the concentration of the paramagnetic center in mM, and 1/T2(+) and 1/T2(−) are the transverse relaxation rates (sec−1) in the presence and absence of the nanoparticle, respectively. C is typically expressed as the concentration of paramagnetic metal, but it can also be expressed as the concentration of NPs or MPs in solution. Here the R2 per metal is multiplied times the number of paramagnetic metal atoms per particle. Magnetic particles with larger numbers of metals per particle are more potent in MRSw assays, see below.
2.2. Magnetic Particles
Magnetic particles can be categorized by their size, with nanoparticles (NPs) being between 10 and 300 nm in diameter, while larger magnetic particles (MPs) are between 300 and 5,000 nm in diameter. Since the first publication demonstrating the MRSw assay principle in 2001 , NPs with surfaces of cross-linked iron oxide (CLIO) have been used for sensing for analytes ranging from small molecules to mammalian cells [5,9–12]. CLIO is an excellent NP both for in vivo MR imaging  and for MRSw assay applications, because of its stability in a variety of fluids, including aqueous buffers and blood, and because of its functional handle of amino groups. CLIO is prepared by two-step treatment of the monocrystalline iron oxide nanoparticle known as MION. The MION NP features a dextran coating which is first cross-linked with epichlorohydrin and then reacted with ammonia to obtain amino groups on the crosslinked dextran surface. MION and CLIO NPs have an iron oxide cores of about 5 nm in diameter and dextran shell (or crosslinked dextran shell) about 10 nm in thickness, so that both NPs have overall diameters between 25 nm and 30 nm.
Recently, magnetic NPs and MPs with improved magnetic properties, and higher detectability per particle, have been described for use with in vivo MR imaging and in vitro biosensor applications [1,14,15]. One strategy is to increase the R2 relaxivity of NPs by increasing M or d, since R2 is proportional to M2d2. Here M is the saturation magnetization per mole of metal or per gram of metal atoms within the particle and d is the particle diameter. [16–18]. Core/shell NPs have been designed with Fe metal cores (not iron oxide cores) and these have an increased Ms and a thin iron oxide shell to block oxidation metal oxidation. They show an enhanced sensitivity compared to CLIO for the detection of bacterial cells . Another strategy employs Mn-doped metal oxide NPs; these also have high Ms and high R2s, and have been synthesized with sizes of 10, 12 and 16 nm. These NPs have been used in the sensitive detection of unprocessed cancer cells, with as few as two cells per 1 μL being detected with miniaturized relaxometer . Another approach to improving the sensitivity of MRSw assays is the use of MPs rather than NPs. These MPs have far more metal atoms per particle than NPs and a far larger per magnetic moments per particle, even though their values or M per metal are typical of older NPs [6,19]. In an MRSw assay of immunoreactive antibodies to influenza, MPs of 1 μm in diameter were employed that had a similar R2 relaxivity to CLIO NPs on a per iron atom basis. However, the larger MPs had 350,000 fold more irons per particle than CLIO NPs. In the MRSw assay for anti-Tag peptide antibody, MPs had 186,000 fold enhanced sensitivity (relative to CLIO). The improvement in sensitivity was achieved by a combination of factors including the use of the larger MP, magnetic field-assisted aggregation of MPs, and valency enhancement achieved by the addition of a secondary antibody . Figure 2B provides a schematic version of the improvement in assay sensitivity shows these techniques.
The stability of NPs or MPs in solution is another important factor in selecting materials for use in MRSw assay applications. Stabilization can be achieved by charge effects leading to electrostatic repulsion between particles or by the use of hydrophilic polymeric coatings that block particle/particle aggregation . Coatings of polymeric dextran make NPs extremely stable and therefore suitable for both in vivo MR and in vitro MRSw assay applications . Attachment of 10 kDa polyethylene glycol (PEG) diamine on the surface of MPs exchanged the initial electrostatic stability of the negatively charged MPs to polymer-based stability and was necessary to use the MPs in MRSw applications . Table 1 reviews the magnetic particles used in MRSw biosensing applications.
Point of care (POC) sensors would benefit home users, clinicians and physicians, and aid in the preparations for bio-warfare and pandemics. The miniaturization of MR relaxometers holds great promise for use as instrumentation with POC [10,16,17,21].
The MR relaxometers used for MRSw assays have three basic components, a magnet, a coil, and a transceiver. Currently MRSw assays depend on the commercial bench top relaxometers such as the 0.47 T Minispec, 20 MHz instrument made by Bruker, Billerica, MA [5, 19]. High throughput MRSw assays have been demonstrated in 384-well plates through the use of a 1.5 T MR scanner [5,22,23]. However, the relaxometer and MR scanner above are impractical as POC sensors due to their high cost, which results principally from the large magnets employed and lack of miniaturized electronic components .
The magnets used in relaxometers can be relatively weak (0.1 to 0.5 T) and can provide less homogeneous magnetic fields than those used in MR imagers. One of the first miniaturized MR relaxometry systems consisted of a small palm-sized permanent magnet and on-board NMR electronics and planar microcoils with integrated microfluidic channels  (see Figure 3A). A multiplexed detection of biomarkers was achieved using an 8 microcoil array and demonstrated the potential application of the microNMR system for high throughput MRSw assays. Optimization of circuit designing in development of RF transceiver integrated circuits led to a small but complete NMR system  (Figure 3B).
2.4. Applications of Type I and Type II MRSw's
As discussed above, magnetic particle aggregation induces T2 changes that are opposite in direction for NPs (T2 decrease, type I MRSw) and MPs (T2 increase, type II, MRSw). Applications of the different types of MRSw systems are discussed below.
2.4.1. Type I MRSw
The amino CLIO NP is a versatile NP for MRSw applications because it is sufficiently stable to permit a variety of surface chemistries [24–26]. CLIO surfaces have been designed to detect ions , DNA [4,5,27], proteins [5,9,19], and cells  such as bacteria and mammalian cells. A particularly valuable system for the study of MRSws is the reaction of NPs displaying the Tag peptide and reacting to a monoclonal antibody (anti Tag) binding to the peptide . The formation of NP aggregates with anti-Tag antibodies has been shown to be analogous to the interactions between antibodies and antigens, with a maximum complex formation occurring at the equivalence point as the concentration of analyte was increased.
A variation of the type I MRSw aggregation/dispersion method is found with the miniaturized NMR system (DMR: diagnostic magnetic resonance). This system achieves a high assay sensitivity by reducing a sample volume to 5 μL and by using filtration methods . Incorporation of microfluidic system with a filter unit into the miniaturized NMR system permitted the detection of bacteria, with as few as 20 colony-forming units per mL of sputum being detected . The size discrepancy between target bacteria and NP probes allowed filter-based concentration of NP-bound bacteria while filtering out unbound NPs.
Another important application of the type I MRSw assay system is its use in an implantable MR based, water relaxation sensor. A semi-permeable membrane was employed with a size cutoff that permitted small analytes, like glucose, to diffuse in and out while the larger CLIO NPs were retained within the sensor . Continuous monitoring of the T2 values of the solution inside the membrane showed a competitive assay type-response of glucose-functionalized CLIO to glucose [23,25]. The proof of concept sensing obtained with glucose was translated to an implantable water relaxation sensor detecting hCG as a cancer biomarker [28,29]. The implantable device had a reservoir that was covered with a semi-permeable polycarbonate membrane and contained CLIO functionalized with antibodies to the hCG cancer biomarker. In vivo MR imaging was used to monitor the T2 values from inside the sensor device. When implanted in a tumor bearing mouse model, the MR signal from the sensor showed significant decreases in 1-4 days due to diffusion of the cancer biomarker hCG into the reservoir and the resulting aggregation of the CLIO NP.
2.4.2. Type II MRSw
Type II MRSws, where biomolecules are attached to MPs and aggregated by reaction with molecular targets, exhibit an increased T2 when aggregated by reaction with a target analyte. With their greater numbers of iron atoms per particle, MPs can be used at concentrations far below than that of NPs in MRSw assays. With the lower concentration of MPs, lower concentrations of analyte are needed to induce aggregation and this results in greatly improved sensitivity [6,19].
When placed in a homogeneous magnetic field, MPs with charge-based or polymer layer-based stability, will aggregate, while NPs will not respond in this fashion [6,7,19,30–33]. The magnetic field-induced MP aggregation is lost when the magnetic field was removed and Brownian effects break down aggregates. The rate of self-assembly formation of MPs in a magnetic field is a function of viscosity and can be used to make a T2 based viscometer. See Figure 2A and . Recently, magnetic field-induced MP aggregation has also been used to accelerate analyte-mediated formation of MP aggregates [19,31,34]. The applied magnetic field enhanced the kinetics of molecular interactions between multivalent analytes, (e.g., a monoclonal Tag antibody), and multivalent MPs displaying the Tag peptide. This technique is referred to as magnetic field enhanced target aggregation and shown in Figure 2B, frame (b).
3. Magnetic Particle Relaxation-Based Sensors
The relaxation of the magnetic moments within magnetic particles have been used as a basis for magnetic particle-based assays.
Magnetic particles in a liquid, with magnetic moments aligned by an applied magnetic field, employ two relaxation mechanisms when magnetic field is turned off: (i) Brownian relaxation and (ii) Néel relaxation. Brownian relaxation is governed by the physical rotation of the entire particle and characterized by the Brownian relaxation time, τB. Here:where VH is the hydrodynamic volume, η is the viscosity of the medium, k is the Boltzmann's constant, and T is the absolute temperature. The monodomain magnetic particle has an anisotropy energy, Ea, which is proportional to the crystal volume.where Ka is the anisotropy constant and V is the volume of the crystal. When the applied field is removed, the magnetization vector within the particle returns to the lowest energy state along the easy axis with a characteristic Néel relaxation time, τN: where τ0 is the preexponential factor that decreases as the anisotropy energy increases. Note that τN is an exponential function of the anisotropy energy that is proportional to the crystal volume.
The effective relaxation rate is expressed as the sum of the Brownian relaxation rate and the Néel relaxation rate:
As Equation (5) shows, faster relaxation time between the two governs the effective relaxation process. Target induced aggregation can decrease the rates of the Brownian or Neel relaxations and this assays for molecular targets are generated. See .
3.2.1. Néel Relaxation Sensors
Superconducting Quantum Interference Devices (SQUIDs) have been used for measurements of the relaxation of particle magnetic moments. The Brownian relaxation is much faster than the Néel relaxation. For a 20 nm single domain magnetite particle in solution, the calculated relaxation times were τB ∼1 μs and τN ∼1 s . The difference in the relaxation time scales was a basis for a homogeneous immunoassay  and a bacterial detection . See Figure 4. The Brownian relaxation time scale of a single unbound magnetic particle was so short, it was out of the detectable range between 1 ms and 1 s of the SQUID. The free Brownian rotation of particles was then restricted when the magnetic particles bound a bacterium. The Néel relaxation was within the detection window of a SQUID, which was used to determine the relaxation time of surface bound particles. The SQUID-based detection of the Néel relaxation time showed a limit of detection of 5 × 104 NPs for a substrate based assay and 1.1 × 105 bacteria in a 20 μL sample volume. Development of a gradiometer instead of a magnetometer suggested a two-order improvement in sensitivity was possible .
3.2.2. Brownian Relaxation Sensors
Measurements of static and dynamic magnetic susceptibility using alternate currents (ac) have permitted use of the Brownian relaxation of NPs for biosensing. As Equation (2) suggests, the NP aggregates that form in recognition of target analytes have a larger hydrodynamic size and thus show slower Brownian relaxation responses than a single NP. The resulting decrease in relaxation was sensed in buffer , and in serum  by using a SQUID or an ac magnetosusceptometer .
Conolly and St. Pierre proposed using the dynamic magnetic properties of NPs in assays . A complex magnetic susceptibility expresses the response of NPs as a function of an alternating magnetic field. According to their theory, the imaginary part has a peak when the frequency equals the inverse of the effective magnetic relaxation time . Brownian relaxation is the dominant relaxation process for these NP based assays. When NPs form molecular target induced aggregates, the hydrodynamic radius is increased and thus decrease of the peak frequency is observed. An antibody was detected at the sensitivity of 0.05 μg/mL (= 0.3 nM) with an AC susceptometer . Recently a volume amplified magnetic nanobead assay showed a dynamic magnetic property-based detection of DNA detection, albeit with an amplification strategy . Here the presence of two different size NPs in a sample provided a high detection of target DNA molecules following a deconvolution of the magnetization data .
4. Magnetoresistive Sensors
Magnetoresistive sensors are based on the binding of magnetic particles to a sensor surface and the magnetic fields of the particles alter the magnetic fields of the sensor which result in electrical current changes within the sensor. There are two mechanisms through which magnetic particles bind to the sensor surface: (i) direct labeling and (ii) indirect labeling (a sandwich type binding). Magnetic probes bind to the surface functionality on the surface in direct labeling by using streptavidin-biotin interaction or complementary DNA sequence recognition. Indirect labeling uses the principle of sandwich immunoassay in ELISA. For example, antibodies that bind to the target protein are immobilized on the surface. After treatment of the surface with a sample solution containing the target proteins, second antibodies that are biotinylated are added to the system. Finally Streptavidin coated magnetic particles are applied for tagging the biotinylated antibodies.
Giant magnetoresistance (GMR) spin valve (SV) or magnetic tunnel junction (MTJ) sensors have been successfully used to sense MPs. Sensors are composed of multiple layers of ferromagnetic materials. A biologically active molecule can be deposited on an Au layer or SiO2 layer to obtain a surface for the attachment of biomolecules. For a review of the structure of magnetoresistive sensors see .
Superparamagnetic particles with different sizes have been used in magnetoresistive biosensing. Earlier applications used relatively large magnetic particles, with diameters between 0.1 and 3 μm . Micrometer sized particles have the advantages of facile observation under light microscope and a higher particle-based magnetic moment that permits detection very small numbers of particles. However, recently magnetic NPs have replaced the larger particles because the NPs are stable in suspension and are less prone to particle clustering in an applied magnetic field [45,47–49]. Streptavidin coated MPs were applied to spin valve sensors in the protein marker detection at 27 pg/mL level of sensitivity . By using 50 nm MACS magnetic nanoparticles, Wang and collaborators demonstrated cancer marker detection in 50% serum at sub picomolar concentrations  (Figure 5).
Improvement of spin valve sensors was achieved by reducing the passivation layer to 30 nm and led to an enhanced sensitivity. A signal amplification strategy that had multiple layers of streptavidin coated NPs and biotinylated antibodies in the sandwich type immunoassay also showed enhanced signals. Multiplex sensing of different protein markers in serum was demonstrated on a single chip by carefully selecting antibodies and by employing the signal enhancing strategy with multiple layers of NPs. Wang and his group in Standford University used nanoimprint lithography to synthesize antiferromagnetic nanoparticles of 100 nm size with high magnetic moment and zero remanence . The antiferromagnetic nanoparticles that have a disk shape were composed of multiple layers of ferromagnetic material separated by a nonmagnetic interlayer. NPs with high magnetic moments were functionalized with streptavidin and permitted the detection of DNA at concentrations as low as 10 pM .
Another effort to synthesize magnetic nanoparticles with high magnetic moment utilized cubic-shaped FeCo nanoparticles of 12.8 nm in a GMR based sensor . The cubic nanoparticles were surface functionalized with silane chemistry for attachment of Streptavidin or antibody. Direct labeling of biotinylated surface with Streptavidin coated nanoparticles allowed detection of 600 nanoparticle binding. Indirect labeling in ELISA type assay produced signals as low as 2 × 106 molecules of a biomarker protein.
See Table 2 for a review of assay configurations and the sensitivities reported for them in the literature.
Magnetic NPs and MPs have been used in different types of biosensors based on different physical principles. Some achieve high sensitivity and, with rapid advances in instrumentation, maybe useful as point-of-care sensors. The continued rapid development of sensors using magnetic materials seems assured.
This work was supported in part by NIH grants U54 CA119349 and P50 CA08635.
- Lee, J.H.; Huh, Y.M.; Jun, Y.W.; Seo, J.W.; Jang, J.T.; Song, H.T.; Kim, S.; Cho, E.J.; Yoon, H.G.; Suh, J.S.; Cheon, J. Artificially engineered magnetic nanoparticles for ultra-sensitive molecular imaging. Nat. Med. 2007, 13, 95–99. [Google Scholar]
- Weissleder, R.; Pittet, M.J. Imaging in the era of molecular oncology. Nature 2008, 452, 580–589. [Google Scholar]
- Weissleder, R. Molecular imaging in cancer. Science 2006, 312, 1168–1171. [Google Scholar]
- Josephson, L.; Perez, J.M.; Weissleder, R. Magnetic nanosensors for the detection of oligonucleotide sequences. Angew. Chem. Int. Ed. 2001, 40, 3204–3208. [Google Scholar]
- Perez, J.M.; Josephson, L.; O'Loughlin, T.; Hogemann, D.; Weissleder, R. Magnetic relaxation switches capable of sensing molecular interactions. Nat. Biotechnol. 2002, 20, 816–820. [Google Scholar]
- Koh, I.; Hong, R.; Weissleder, R.; Josephson, L. Nanoparticle-target interactions parallel antibody-protein interactions. Anal. Chem. 2009, 81, 3618–3622. [Google Scholar]
- Hong, R.; Cima, M.J.; Weissleder, R.; Josephson, L. Magnetic microparticle aggregation for viscosity determination by MR. Magn. Reson. Med. 2008, 59, 515–520. [Google Scholar]
- Laurent, S.; Forge, D.; Port, M.; Roch, A.; Robic, C.; Vander Elst, L.; Muller, R.N. Magnetic iron oxide nanoparticles: synthesis, stabilization, vectorization, physicochemical characterizations, and biological applications. Chem. Rev. 2008, 108, 2064–2110. [Google Scholar]
- Kim, G.Y.; Josephson, L.; Langer, R.; Cima, M.J. Magnetic relaxation switch detection of human chorionic gonadotrophin. Bioconjug. Chem. 2007, 18, 2024–2028. [Google Scholar]
- Lee, H.; Sun, E.; Ham, D.; Weissleder, R. Chip-NMR biosensor for detection and molecular analysis of cells. Nat. Med. 2008, 14, 869–874. [Google Scholar]
- Perez, J.M.; Simeone, F.J.; Saeki, Y.; Josephson, L.; Weissleder, R. Viral-induced self-assembly of magnetic nanoparticles allows the detection of viral particles in biological media. J. Am. Chem. Soc. 2003, 125, 10192–10193. [Google Scholar]
- Taktak, S.; Weissleder, R.; Josephson, L. Electrode chemistry yields a nanoparticle-based nmr sensor for Calcium. Langmuir 2008, 24, 7596–7598. [Google Scholar]
- Harisinghani, M.G.; Barentsz, J.; Hahn, P.F.; Deserno, W.M.; Tabatabaei, S.; van de Kaa, C.H.; de la Rosette, J.; Weissleder, R. Noninvasive detection of clinically occult lymph-node metastases in prostate cancer. N. Engl. J. Med. 2003, 348, 2491–2499. [Google Scholar]
- Jun, Y.W.; Huh, Y.M.; Choi, J.S.; Lee, J.H.; Song, H.T.; Kim, S.; Yoon, S.; Kim, K.S.; Shin, J.S.; Suh, J.S.; Cheon, J. Nanoscale size effect of magnetic nanocrystals and their utilization for cancer diagnosis via magnetic resonance imaging. J. Am. Chem. Soc. 2005, 127, 5732–5733. [Google Scholar]
- Park, J.; An, K.J.; Hwang, Y.S.; Park, J.G.; Noh, H.J.; Kim, J.Y.; Park, J.H.; Hwang, N.M.; Hyeon, T. Ultra-large-scale syntheses of monodisperse nanocrystals. Nat. Mater. 2004, 3, 891–895. [Google Scholar]
- Lee, H.; Yoon, T.J.; Figueiredo, J.L.; Swirski, F.K.; Weissleder, R. Rapid detection and profiling of cancer cells in fine-needle aspirates. Proc. Natl. Acad. Sci. USA 2009. [Google Scholar] [CrossRef]
- Lee, H.; Yoon, T.J.; Weissleder, R. Ultrasensitive detection of bacteria using core-shell nanoparticles and an NMR-filter system. Angew.Chem. Int. Ed. 2009, 48, 5657–5660. [Google Scholar]
- Park, J.H.; von Maltzahn, G.; Zhang, L.L.; Schwartz, M.P.; Ruoslahti, E.; Bhatia, S.N.; Sailor, M.J. Magnetic iron oxide nanoworms for tumor targeting and imaging. Adv. Mater. 2008, 20, 1630–1635. [Google Scholar]
- Koh, I.; Hong, R.; Weissleder, R.; Josephson, L. Sensitive NMR sensors detect antibodies to influenza. Angew. Chem. Int. Ed. 2008, 47, 4119–4121. [Google Scholar]
- Perez, J.M.; Josephson, L.; Weissleder, R. Use of magnetic nanoparticles as nanosensors to probe for molecular interactions. Chembiochem 2004, 5, 261–264. [Google Scholar]
- Sun, N.; Liu, Y.; Lee, H.; Weissleder, R.; Ham, D. CMOS RF biosensor utilizing nuclear magnetic resonance. IEEE J. Solid-State Circuits 2009, 44, 1629–1643. [Google Scholar]
- Grimm, J.; Perez, J.M.; Josephson, L.; Weissleder, R. Novel nanosensors for rapid analysis of telomerase activity. Cancer Res. 2004, 64, 639–643. [Google Scholar]
- Tsourkas, A.; Hofstetter, O.; Hofstetter, H.; Weissleder, R.; Josephson, L. Magnetic relaxation switch immunosensors detect enantiomeric impurities. Angew. Chem. Int. Ed. 2004, 43, 2395–2399. [Google Scholar]
- Sun, E.Y.; Josephson, L.; Weissleder, R. “Clickable” nanoparticles for targeted imaging. Mol. Imaging 2006, 5, 122–128. [Google Scholar]
- Sun, E.Y.; Weissleder, R.; Josephson, L. Continuous analyte sensing with magnetic nanoswitches. Small 2006, 2, 1144–1147. [Google Scholar]
- Weissleder, R.; Kelly, K.; Sun, E.Y.; Shtatland, T.; Josephson, L. Cell-specific targeting of nanoparticles by multivalent attachment of small molecules. Nat. Biotechnol. 2005, 23, 1418–1423. [Google Scholar]
- Perez, J.M.; O'Loughin, T.; Simeone, F.J.; Weissleder, R.; Josephson, L. DNA-based magnetic nanoparticle assembly acts as a magnetic relaxation nanoswitch allowing screening of DNA-cleaving agents. J. Am. Chem. Soc. 2002, 124, 2856–2857. [Google Scholar]
- Daniel, K.D.; Kim, G.Y.; Vassiliou, C.C.; Galindo, M.; Guimaraes, A.R.; Weissleder, R.; Charest, A.; Langer, R.; Cima, M.J. Implantable diagnostic device for cancer monitoring. Biosens. Bioelectron. 2009, 24, 3252–3257. [Google Scholar]
- Daniel, K.D.; Kim, G.Y.; Vassiliou, C.C.; Jalali-Yazdi, F.; Langer, R.; Cima, M.J. Multi-reservoir device for detecting a soluble cancer biomarker. Lab. Chip. 2007, 7, 1288–1293. [Google Scholar]
- Doyle, P.S.; Bibette, J.; Bancaud, A.; Viovy, J.L. Self-assembled magnetic matrices for DNA separation chips. Science 2002, 295, 2237. [Google Scholar]
- Baudry, J.; Rouzeau, C.; Goubault, C.; Robic, C.; Cohen-Tannoudji, L.; Koenig, A.; Bertrand, E.; Bibette, J. Acceleration of the recognition rate between grafted ligands and receptors with magnetic forces. Proc. Natl. Acad. Sci.USA 2006, 103, 16076–16078. [Google Scholar]
- Singh, H.; Laibinis, P.E.; Hatton, T.A. Rigid, superparamagnetic chains of permanently linked beads coated with magnetic nanoparticles. Synthesis and rotational dynamics under applied magnetic fields. Langmuir 2005, 21, 11500–11509. [Google Scholar]
- Zerrouki, D.; Baudry, J.; Pine, D.; Chaikin, P.; Bibette, J. Chiral colloidal clusters. Nature 2008, 455, 380–382. [Google Scholar]
- Cohen-Tannoudji, L.; Bertrand, E.; Baudry, J.; Robic, C.; Goubault, C.; Pellissier, M.; Johner, A.; Thalmann, F.; Lee, N.K.; Marques, C.M.; Bibette, J. Measuring the kinetics of biomolecular recognition with magnetic colloids. Phys. Rev. Lett. 2008, 100, 108301:1–108301:4. [Google Scholar]
- Chemla, Y.R.; Grossman, H.L.; Poon, Y.; McDermott, R.; Stevens, R.; Alper, M.D.; Clarke, J. Ultrasensitive magnetic biosensor for homogeneous immunoassay. Proc. Natl. Acad. Sci. USA 2000, 97, 14268–14272. [Google Scholar]
- Grossman, H.L.; Myers, W.R.; Vreeland, V.J.; Bruehl, R.; Alper, M.D.; Bertozzi, C.R.; Clarke, J. Detection of bacteria in suspension by using a superconducting quantum interference device. Proc. Natl. Acad. Sci. USA 2004, 101, 129–134. [Google Scholar]
- Lee, S.; Myers, W.R.; Grossman, H.L.; Cho, H.M.; Chemla, Y.R.; Clarke, J. Magnetic gradiometer based on a high-transition temperature superconducting quantum interference device for improved sensitivity of a biosensor. Appl. Phys. Lett. 2002, 81, 3094–3096. [Google Scholar]
- Hong, C.Y.; Wu, C.C.; Chiu, Y.C.; Yang, S.Y.; Horng, H.E.; Yang, H.C. Magnetic susceptibility reduction method for magnetically labeled immunoassay. Appl. Phys. Lett. 2006, 88, 212512:1–212512:3. [Google Scholar]
- Hong, C.Y.; Chen, W.S.; Jian, Z.F.; Yang, S.Y.; Horng, H.E.; Yang, L.C.; Yang, H.C. Wash-free immunomagnetic detection for serum through magnetic susceptibility reduction. Appl. Phys. Lett. 2007, 90, 074105:1–074105:3. [Google Scholar]
- Connolly, J.; St Pierre, T.G. Proposed biosensors based on time-dependent properties of magnetic fluids. J. Magn. Magn. Mater. 2001, 225, 156–160. [Google Scholar]
- Chung, S.H.; Hoffmann, A.; Bader, S.D.; Liu, C.; Kay, B.; Makowski, L.; Chen, L. Biological sensors based on Brownian relaxation of magnetic nanoparticles. Appl. Phys. Lett. 2004, 85, 2971–2973. [Google Scholar]
- Fornara, A.; Johansson, P.; Petersson, K.; Gustafsson, S.; Qin, J.; Olsson, E.; Ilver, D.; Krozer, A.; Muhammed, M.; Johansson, C. Tailored magnetic nanoparticles for direct and sensitive detection of biomolecules in biological samples. Nano Lett. 2008, 8, 3423–3428. [Google Scholar]
- Stromberg, M.; Goransson, J.; Gunnarsson, K.; Nilsson, M.; Svedlindh, P.; Stromme, M. Sensitive molecular diagnostics using volume-amplified magnetic nanobeads. Nano Lett. 2008, 8, 816–821. [Google Scholar]
- Stromberg, M.; Zardan Gomez de la Torre, T.; Goransson, J.; Gunnarsson, K.; Nilsson, M.; Svedlindh, P.; Stromme, M. Multiplex detection of DNA sequences using the volume-amplified magnetic nanobead detection assay. Anal. Chem. 2009, 81, 3398–3406. [Google Scholar]
- Wang, S.X.; Li, G. Advances in giant magnetoresistance biosensors with magnetic nanoparticle tags: Review and outlook. IEEE Trans. Magn. 2008, 44, 1687–1702. [Google Scholar]
- Graham, D.L.; Ferreira, H.A.; Freitas, P.P. Magnetoresistive-based biosensors and biochips. Trends Biotechnol. 2004, 22, 455–462. [Google Scholar]
- Fu, A.; Hu, W.; Xu, L.; Wilson, R.J.; Yu, H.; Osterfeld, S.J.; Gambhir, S.S.; Wang, S.X. Protein-functionalized synthetic antiferromagnetic nanoparticles for biomolecule detection and magnetic manipulation. Angew. Chem. Int. Ed. 2009, 48, 1620–1624. [Google Scholar]
- Osterfeld, S.J.; Yu, H.; Gaster, R.S.; Caramuta, S.; Xu, L.; Han, S.J.; Hall, D.A.; Wilson, R.J.; Sun, S.; White, R.L.; Davis, R.W.; Pourmand, N.; Wang, S.X. Multiplex protein assays based on real-time magnetic nanotag sensing. Proc. Natl. Acad. Sci. USA 2008, 105, 20637–20640. [Google Scholar]
- Srinivasan, B.; Li, Y.; Jing, Y.; Xu, Y.; Yao, X.; Xing, C.; Wang, J.P. A detection system based on giant magnetoresistive sensors and high-moment magnetic nanoparticles demonstrates zeptomole sensitivity: potential for personalized medicine. Angew. Chem. Int. Ed. 2009, 48, 2764–2767. [Google Scholar]
- De Palma, R.; Reekmans, G.; Liu, C.; Wirix-Speetjens, R.; Laureyn, W.; Nilsson, O.; Lagae, L. Magnetic bead sensing platform for the detection of proteins. Anal. Chem. 2007, 79, 8669–8677. [Google Scholar]
- Hu, W.; Wilson, C.R.J.; Koh, A.; Fu, A.H.; Faranesh, A.Z.; Earhart, C.M.; Osterfeld, S.J.; Han, S.J.; Xu, L.; Guccione, S.; Sinclair, R.; Wang, S.X. High-moment antiferromagnetic nanoparticles with tunable magnetic properties. Adv. Mater. 2008, 20, 1479–1483. [Google Scholar]
- Lowery, T.J.; Palazzolo, R.; Wong, S.M.; Prado, P.J.; Taktak, S. Single-coil, multisample, proton relaxation method for magnetic relaxation switch assays. Anal. Chem. 2008, 80, 1118–1123. [Google Scholar]
|Table 1. Characteristics of magnetic particles used for biosensing applications.|
|CLIO||∼30 nm||5 nm core, 10 nm dextran coating||MRSw, R2 = 50 (s·mM Fe)-1|||
|Core/shell||16 nm||Fe core, iron oxide shell, 2.5 nm shell thickness||MRSw, R2 = 260 (s·mM Fe)-1|||
|Mn-MNPa||16 nm||Mn-doped iron oxide||MRSw, R2 = 420 (s·mM metal)-1|||
|MP||1000 nm||Commercial (Dynabeads)||MRSw, R2 = 43 (s·mM Fe)-1|||
|Iron oxide||56 nm||Commercial (Quantum Magnetics, Miltenyi Biotech)||SQUID||[35,36]|
|Iron oxide||19.5 nm||AC susceptometer|||
|Cubic FeCo||12.8 nm||1.5 nm oxidized shell||GMR|||
|SAFb||100 nm||Multilayers of ferromagnetic, interlayer of nonmagnetic material||GMR, disk shape|||
|Magnetic bead||130, 250 nm||Commercial (Micromod Partikeltechnologie)||SQUID|||
(a)MNP: magnetic nanoparticle,(b)SAF: synthetic antiferromagnetic.
|Table 2. Sensitivities of magnetic particle based biosensors.|
|Analyte||Magnetic particle/instrumentation||Sensitivity||Sample volume||Reference|
|MRSw type I||nucleotide||CLIO, bench top relaxometer||Low nM∼pM||300 μL||[4,5]|
|proteins||CLIO, bench top relaxometer||Low nM||300 μL||[5,9]|
|virus||CLIO, MRI||50 viruses/100 μL||100 μL|||
|bacteria||core/shell, DMRa||20 CFUb/100 μL|
|Cancer cell||Mn-MNP, DMR||2 cells/1 μL||5 μL|||
|MRSw Type II||antibody||MP, bench top relaxometer||<1 pM||300 μL|||
|AC susceptometer||antibody||Iron oxide NP||<1 nM|||
|SQUID||bacteria||Iron oxide NP||1.1 × 105 bacteria/20 μL|||
|DNA||Magnetic bead||3∼10 pM|
|GMR||Protein||Cubic FeCo NP||2 × 106 proteins||2 μL|||
|DNA||Antiferromagnetic NP||10 pM|||
|Protein||Iron oxide NP||2.4 pM|||
(a)DMR: diagnostic magnetic resonance,(b)CFU: colony forming unit.
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).