Next Article in Journal
Using LOTOS for FormalisingWireless Sensor Network Applications
Next Article in Special Issue
Hybrid Integrated Silicon Microfluidic Platform for Fluorescence Based Biodetection
Previous Article in Journal
Development and Electrochemical Investigations of an EIS- (Electrolyte-Insulator-Semiconductor) based Biosensor for Cyanide Detection
Previous Article in Special Issue
Raman Spectroscopy Cell-based Biosensors
Article Menu

Export Article

Open AccessArticle
Sensors 2007, 7(8), 1427-1446; doi:10.3390/s7081427

Detection of Salmonella by Surface Plasmon Resonance

1
Institut für Pharmazeutische Chemie, Philipps-Universität Marburg, Marbacher Weg 6, D-35032 Marburg, Germany
2
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Gießen, Heinrich-Buff-Ring 26-32, D-35392 Gießen, Germany
*
Author to whom correspondence should be addressed.
Received: 26 June 2007 / Accepted: 3 August 2007 / Published: 7 August 2007
(This article belongs to the Special Issue Optical Biosensors)
View Full-Text   |   Download PDF [1854 KB, uploaded 21 June 2014]   |  

Abstract

This study explores the possibility of simultaneous and specific detection ofSalmonella serovars by surface plasmon resonance (SPR). The Plasmonic® SPR device wasused to develop this rapid assay. The sandwich immunoassay involves the use of apolyclonal anti-Salmonella antibody to simultaneous capture multiple Salmonella serovarspresent in a sample. This is followed by specific detection of the captured serovars usingO-specific anti-Salmonella antibodies. Milk spiked with Salmonella Typhimurium andSalmonella Enteritidis was used as a model system to establish the assay. The assay wasfurther extended to sequentially differentiate between the two Salmonella serovars on asingle SPR chip in a single channel. The assay was proved to work without any additionaldilution or clean-up steps. The sample volume requirement for the assay is only 10 μL. Thelower limits of detection for Salmonella Typhimurium and Salmonella Enteritidis were2.50×105 cells mL-1 and 2.50×108 cells mL-1, respectively.
Keywords: Salmonella serovars; lipopolysaccharide (LPS); O-specific antibody; sequential detection; Plasmonic® surface plasmon resonance (SPR) devic Salmonella serovars; lipopolysaccharide (LPS); O-specific antibody; sequential detection; Plasmonic® surface plasmon resonance (SPR) devic
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Barlen, B.; Mazumdar, S.D.; Lezrich, O.; Kämpfer, P.; Keusgen, M. Detection of Salmonella by Surface Plasmon Resonance. Sensors 2007, 7, 1427-1446.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top