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Detection of Salmonella by Surface Plasmon Resonance
Institut für Pharmazeutische Chemie, Philipps-Universität Marburg, Marbacher Weg 6, D-35032 Marburg, Germany
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Gießen, Heinrich-Buff-Ring 26-32, D-35392 Gießen, Germany
* Author to whom correspondence should be addressed.
Received: 26 June 2007; Accepted: 3 August 2007 / Published: 7 August 2007
Abstract: This study explores the possibility of simultaneous and specific detection ofSalmonella serovars by surface plasmon resonance (SPR). The Plasmonic® SPR device wasused to develop this rapid assay. The sandwich immunoassay involves the use of apolyclonal anti-Salmonella antibody to simultaneous capture multiple Salmonella serovarspresent in a sample. This is followed by specific detection of the captured serovars usingO-specific anti-Salmonella antibodies. Milk spiked with Salmonella Typhimurium andSalmonella Enteritidis was used as a model system to establish the assay. The assay wasfurther extended to sequentially differentiate between the two Salmonella serovars on asingle SPR chip in a single channel. The assay was proved to work without any additionaldilution or clean-up steps. The sample volume requirement for the assay is only 10 μL. Thelower limits of detection for Salmonella Typhimurium and Salmonella Enteritidis were2.50×105 cells mL-1 and 2.50×108 cells mL-1, respectively.
Keywords: Salmonella serovars; lipopolysaccharide (LPS); O-specific antibody; sequential detection; Plasmonic® surface plasmon resonance (SPR) devic
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Barlen, B.; Mazumdar, S.D.; Lezrich, O.; Kämpfer, P.; Keusgen, M. Detection of Salmonella by Surface Plasmon Resonance. Sensors 2007, 7, 1427-1446.
Barlen B, Mazumdar SD, Lezrich O, Kämpfer P, Keusgen M. Detection of Salmonella by Surface Plasmon Resonance. Sensors. 2007; 7(8):1427-1446.
Barlen, Benjamin; Mazumdar, Saikat D.; Lezrich, Olga; Kämpfer, Peter; Keusgen, Michael. 2007. "Detection of Salmonella by Surface Plasmon Resonance." Sensors 7, no. 8: 1427-1446.