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Sensors 2007, 7(8), 1427-1446; doi:10.3390/s7081427
Article

Detection of Salmonella by Surface Plasmon Resonance

1
, 1
, 1
, 2
 and 1,*
Received: 26 June 2007; Accepted: 3 August 2007 / Published: 7 August 2007
(This article belongs to the Special Issue Optical Biosensors)
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Abstract: This study explores the possibility of simultaneous and specific detection ofSalmonella serovars by surface plasmon resonance (SPR). The Plasmonic® SPR device wasused to develop this rapid assay. The sandwich immunoassay involves the use of apolyclonal anti-Salmonella antibody to simultaneous capture multiple Salmonella serovarspresent in a sample. This is followed by specific detection of the captured serovars usingO-specific anti-Salmonella antibodies. Milk spiked with Salmonella Typhimurium andSalmonella Enteritidis was used as a model system to establish the assay. The assay wasfurther extended to sequentially differentiate between the two Salmonella serovars on asingle SPR chip in a single channel. The assay was proved to work without any additionaldilution or clean-up steps. The sample volume requirement for the assay is only 10 μL. Thelower limits of detection for Salmonella Typhimurium and Salmonella Enteritidis were2.50×105 cells mL-1 and 2.50×108 cells mL-1, respectively.
Keywords: Salmonella serovars; lipopolysaccharide (LPS); O-specific antibody; sequential detection; Plasmonic® surface plasmon resonance (SPR) devic Salmonella serovars; lipopolysaccharide (LPS); O-specific antibody; sequential detection; Plasmonic® surface plasmon resonance (SPR) devic
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Barlen, B.; Mazumdar, S.D.; Lezrich, O.; Kämpfer, P.; Keusgen, M. Detection of Salmonella by Surface Plasmon Resonance. Sensors 2007, 7, 1427-1446.

AMA Style

Barlen B, Mazumdar SD, Lezrich O, Kämpfer P, Keusgen M. Detection of Salmonella by Surface Plasmon Resonance. Sensors. 2007; 7(8):1427-1446.

Chicago/Turabian Style

Barlen, Benjamin; Mazumdar, Saikat D.; Lezrich, Olga; Kämpfer, Peter; Keusgen, Michael. 2007. "Detection of Salmonella by Surface Plasmon Resonance." Sensors 7, no. 8: 1427-1446.



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