• Submit to Sensors Login Register
• MDPI
• Journals A-Z
• For Authors
• For Editors
• About
• Open Access Policy

• Abstract
• Full-Text PDF [1854 KB]

• Article Statistics
• Google Scholar
• Order Reprints

• Cite this article
• Export to BibTeX
• Export to EndNote

• [+] on DOAJ
• Barlen, B
• Mazumdar, SDatta
• Lezrich, O
• Kämpfer, P
• Keusgen, M
• [+] on Google Scholar
• Barlen, B
• Mazumdar, SDatta
• Lezrich, O
• Kämpfer, P
• Keusgen, M
• [+] on PubMed
• Barlen, B
• Mazumdar, SDatta
• Lezrich, O
• Kämpfer, P
• Keusgen, M

•  CiteULike
•  Facebook
•  Mendeley
•  Twitter

This article is

• freely available
• re-usable

Sensors 2007, 7(8), 1427-1446; doi:10.3390/s7081427

Article

Detection of Salmonella by Surface Plasmon Resonance

Benjamin Barlen ¹ , Saikat Datta Mazumdar ¹ , Olga Lezrich ¹ , Peter Kämpfer ²  and Michael Keusgen ^1,*

¹ Institut für Pharmazeutische Chemie, Philipps-Universität Marburg, Marbacher Weg 6, D-35032 Marburg, Germany ² Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Gießen, Heinrich-Buff-Ring 26-32, D-35392 Gießen, Germany

* Author to whom correspondence should be addressed.

Received: 26 June 2007 / Accepted: 3 August 2007 / Published: 7 August 2007

(This article belongs to the Special Issue Optical Biosensors)

Download PDF Full-Text [1854 KB, uploaded 20 June 2008 16:52 CEST]

Abstract: This study explores the possibility of simultaneous and specific detection ofSalmonella serovars by surface plasmon resonance (SPR). The Plasmonic^® SPR device wasused to develop this rapid assay. The sandwich immunoassay involves the use of apolyclonal anti-Salmonella antibody to simultaneous capture multiple Salmonella serovarspresent in a sample. This is followed by specific detection of the captured serovars usingO-specific anti-Salmonella antibodies. Milk spiked with Salmonella Typhimurium andSalmonella Enteritidis was used as a model system to establish the assay. The assay wasfurther extended to sequentially differentiate between the two Salmonella serovars on asingle SPR chip in a single channel. The assay was proved to work without any additionaldilution or clean-up steps. The sample volume requirement for the assay is only 10 μL. Thelower limits of detection for Salmonella Typhimurium and Salmonella Enteritidis were2.50×10⁵ cells mL^-1 and 2.50×10⁸ cells mL^-1, respectively.

Keywords: Salmonella serovars; lipopolysaccharide (LPS); O-specific antibody; sequential detection; Plasmonic® surface plasmon resonance (SPR) devic

Article Statistics

Cite This Article