Sensors 2007, 7(8), 1415-1426; doi:10.3390/s7081415
Article

Development and Electrochemical Investigations of an EIS- (Electrolyte-Insulator-Semiconductor) based Biosensor for Cyanide Detection

Received: 16 July 2007; Accepted: 3 August 2007 / Published: 3 August 2007
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: A cyanide biosensor based on a pH-sensitive p-doped electrolyte-insulator-semiconductor (EIS) structure with an immobilised enzyme (cyanidase) is realised at thelaboratory scale. The immobilisation of the cyanidase is performed in two distinct steps:first, the covalent coupling of cyanidase to an N-hydroxysuccinimide- (NHS) activatedSepharoseTM gel and then, the physical entrapment of NHS-activated SepharoseTM with theimmobilised cyanidase in a dialysis membrane onto the EIS structure. The immobilisationof the cyanidase to the NHS-activated SepharoseTM is studied by means of gelelectrophoresis measurements and investigations using an ammonia- (NH3) selectiveelectrode. For the electrochemical characterisation of the cyanide biosensor,capacitance/voltage and constant capacitance measurements, respectively, have beencarried out. A differential measurement procedure is presented to evaluate the cyanideconcentration-dependent biosensor signals.
Keywords: cyanide biosensor; EIS structure; cyanidase; pH; differential measurement set- up.
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MDPI and ACS Style

Turek, M.; Ketterer, L.; Claßen, M.; Berndt, H.K.; Elbers, G.; Krüger, P.; Keusgen, M.; Schöning, M.J. Development and Electrochemical Investigations of an EIS- (Electrolyte-Insulator-Semiconductor) based Biosensor for Cyanide Detection. Sensors 2007, 7, 1415-1426.

AMA Style

Turek M, Ketterer L, Claßen M, Berndt HK, Elbers G, Krüger P, Keusgen M, Schöning MJ. Development and Electrochemical Investigations of an EIS- (Electrolyte-Insulator-Semiconductor) based Biosensor for Cyanide Detection. Sensors. 2007; 7(8):1415-1426.

Chicago/Turabian Style

Turek, Monika; Ketterer, Lothar; Claßen, Melanie; Berndt, Heinz K.; Elbers, Gereon; Krüger, Peter; Keusgen, Michael; Schöning, Michael J. 2007. "Development and Electrochemical Investigations of an EIS- (Electrolyte-Insulator-Semiconductor) based Biosensor for Cyanide Detection." Sensors 7, no. 8: 1415-1426.

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