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Hybrid Integrated Silicon Microfluidic Platform for Fluorescence Based Biodetection
CONCAVE Research Center, Optical Microsystems Laboratory, Dept. of Mechanical Engineering, CONCORDIA University, 1515 St. Catherine (o), Montreal, QC H3G2W1, Canada
Department of Biochemistry and Microbiology, University of Laval, Quebec, Canada
* Author to whom correspondence should be addressed.
Received: 24 August 2007; Accepted: 10 September 2007 / Published: 11 September 2007
Abstract: The desideratum to develop a fully integrated Lab-on-a-chip device capable ofrapid specimen detection for high throughput in-situ biomedical diagnoses and Point-of-Care testing applications has called for the integration of some of the novel technologiessuch as the microfluidics, microphotonics, immunoproteomics and Micro ElectroMechanical Systems (MEMS). In the present work, a silicon based microfluidic device hasbeen developed for carrying out fluorescence based immunoassay. By hybrid attachment ofthe microfluidic device with a Spectrometer-on-chip, the feasibility of synthesizing anintegrated Lab-on-a-chip type device for fluorescence based biosensing has beendemonstrated. Biodetection using the microfluidic device has been carried out usingantigen sheep IgG and Alexafluor-647 tagged antibody particles and the experimentalresults prove that silicon is a compatible material for the present application given thevarious advantages it offers such as cost-effectiveness, ease of bulk microfabrication,superior surface affinity to biomolecules, ease of disposability of the device etc., and is thussuitable for fabricating Lab-on-a-chip type devices.
Keywords: Micro-Opto-Electro-Mechanical Systems (MOEMS); Lab-on-a-chip; Biosensing; Immobilization; Microfluidics; Anisotropic etching; Fluorescence; Spectrometer-on-chip.
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Chandrasekaran, A.; Acharya, A.; You, J.L.; Soo, K.Y.; Packirisamy, M.; Stiharu, I.; Darveau, A. Hybrid Integrated Silicon Microfluidic Platform for Fluorescence Based Biodetection. Sensors 2007, 7, 1901-1915.
Chandrasekaran A, Acharya A, You JL, Soo KY, Packirisamy M, Stiharu I, Darveau A. Hybrid Integrated Silicon Microfluidic Platform for Fluorescence Based Biodetection. Sensors. 2007; 7(9):1901-1915.
Chandrasekaran, Arvind; Acharya, Ashwin; You, Jian L.; Soo, Kim Y.; Packirisamy, Muthukumaran; Stiharu, Ion; Darveau, André. 2007. "Hybrid Integrated Silicon Microfluidic Platform for Fluorescence Based Biodetection." Sensors 7, no. 9: 1901-1915.