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Sensors 2007, 7(9), 1901-1915; doi:10.3390/s7091901
Article

Hybrid Integrated Silicon Microfluidic Platform for Fluorescence Based Biodetection

1,* , 1, 1, 1, 1, 1 and 2
1 CONCAVE Research Center, Optical Microsystems Laboratory, Dept. of Mechanical Engineering, CONCORDIA University, 1515 St. Catherine (o), Montreal, QC H3G2W1, Canada 2 Department of Biochemistry and Microbiology, University of Laval, Quebec, Canada
* Author to whom correspondence should be addressed.
Received: 24 August 2007 / Accepted: 10 September 2007 / Published: 11 September 2007
(This article belongs to the Special Issue Optical Biosensors)

Abstract

The desideratum to develop a fully integrated Lab-on-a-chip device capable ofrapid specimen detection for high throughput in-situ biomedical diagnoses and Point-of-Care testing applications has called for the integration of some of the novel technologiessuch as the microfluidics, microphotonics, immunoproteomics and Micro ElectroMechanical Systems (MEMS). In the present work, a silicon based microfluidic device hasbeen developed for carrying out fluorescence based immunoassay. By hybrid attachment ofthe microfluidic device with a Spectrometer-on-chip, the feasibility of synthesizing anintegrated Lab-on-a-chip type device for fluorescence based biosensing has beendemonstrated. Biodetection using the microfluidic device has been carried out usingantigen sheep IgG and Alexafluor-647 tagged antibody particles and the experimentalresults prove that silicon is a compatible material for the present application given thevarious advantages it offers such as cost-effectiveness, ease of bulk microfabrication,superior surface affinity to biomolecules, ease of disposability of the device etc., and is thussuitable for fabricating Lab-on-a-chip type devices.
Keywords: Micro-Opto-Electro-Mechanical Systems (MOEMS); Lab-on-a-chip; Biosensing; Immobilization; Microfluidics; Anisotropic etching; Fluorescence; Spectrometer-on-chip. Micro-Opto-Electro-Mechanical Systems (MOEMS); Lab-on-a-chip; Biosensing; Immobilization; Microfluidics; Anisotropic etching; Fluorescence; Spectrometer-on-chip.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Chandrasekaran, A.; Acharya, A.; You, J.L.; Soo, K.Y.; Packirisamy, M.; Stiharu, I.; Darveau, A. Hybrid Integrated Silicon Microfluidic Platform for Fluorescence Based Biodetection. Sensors 2007, 7, 1901-1915.

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