Abstract: The desideratum to develop a fully integrated Lab-on-a-chip device capable ofrapid specimen detection for high throughput in-situ biomedical diagnoses and Point-of-Care testing applications has called for the integration of some of the novel technologiessuch as the microfluidics, microphotonics, immunoproteomics and Micro ElectroMechanical Systems (MEMS). In the present work, a silicon based microfluidic device hasbeen developed for carrying out fluorescence based immunoassay. By hybrid attachment ofthe microfluidic device with a Spectrometer-on-chip, the feasibility of synthesizing anintegrated Lab-on-a-chip type device for fluorescence based biosensing has beendemonstrated. Biodetection using the microfluidic device has been carried out usingantigen sheep IgG and Alexafluor-647 tagged antibody particles and the experimentalresults prove that silicon is a compatible material for the present application given thevarious advantages it offers such as cost-effectiveness, ease of bulk microfabrication,superior surface affinity to biomolecules, ease of disposability of the device etc., and is thussuitable for fabricating Lab-on-a-chip type devices.
Keywords: Micro-Opto-Electro-Mechanical Systems (MOEMS); Lab-on-a-chip; Biosensing; Immobilization; Microfluidics; Anisotropic etching; Fluorescence; Spectrometer-on-chip.
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Chandrasekaran, A.; Acharya, A.; You, J.L.; Soo, K.Y.; Packirisamy, M.; Stiharu, I.; Darveau, A. Hybrid Integrated Silicon Microfluidic Platform for Fluorescence Based Biodetection. Sensors 2007, 7, 1901-1915.
Chandrasekaran A, Acharya A, You JL, Soo KY, Packirisamy M, Stiharu I, Darveau A. Hybrid Integrated Silicon Microfluidic Platform for Fluorescence Based Biodetection. Sensors. 2007; 7(9):1901-1915.
Chandrasekaran, Arvind; Acharya, Ashwin; You, Jian L.; Soo, Kim Y.; Packirisamy, Muthukumaran; Stiharu, Ion; Darveau, André. 2007. "Hybrid Integrated Silicon Microfluidic Platform for Fluorescence Based Biodetection." Sensors 7, no. 9: 1901-1915.