Sensors 2007, 7(9), 1901-1915; doi:10.3390/s7091901

Hybrid Integrated Silicon Microfluidic Platform for Fluorescence Based Biodetection

1,* email, 1, 1, 1, 1, 1 and 2email
Received: 24 August 2007; Accepted: 10 September 2007 / Published: 11 September 2007
(This article belongs to the Special Issue Optical Biosensors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The desideratum to develop a fully integrated Lab-on-a-chip device capable ofrapid specimen detection for high throughput in-situ biomedical diagnoses and Point-of-Care testing applications has called for the integration of some of the novel technologiessuch as the microfluidics, microphotonics, immunoproteomics and Micro ElectroMechanical Systems (MEMS). In the present work, a silicon based microfluidic device hasbeen developed for carrying out fluorescence based immunoassay. By hybrid attachment ofthe microfluidic device with a Spectrometer-on-chip, the feasibility of synthesizing anintegrated Lab-on-a-chip type device for fluorescence based biosensing has beendemonstrated. Biodetection using the microfluidic device has been carried out usingantigen sheep IgG and Alexafluor-647 tagged antibody particles and the experimentalresults prove that silicon is a compatible material for the present application given thevarious advantages it offers such as cost-effectiveness, ease of bulk microfabrication,superior surface affinity to biomolecules, ease of disposability of the device etc., and is thussuitable for fabricating Lab-on-a-chip type devices.
Keywords: Micro-Opto-Electro-Mechanical Systems (MOEMS); Lab-on-a-chip; Biosensing; Immobilization; Microfluidics; Anisotropic etching; Fluorescence; Spectrometer-on-chip.
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MDPI and ACS Style

Chandrasekaran, A.; Acharya, A.; You, J.L.; Soo, K.Y.; Packirisamy, M.; Stiharu, I.; Darveau, A. Hybrid Integrated Silicon Microfluidic Platform for Fluorescence Based Biodetection. Sensors 2007, 7, 1901-1915.

AMA Style

Chandrasekaran A, Acharya A, You JL, Soo KY, Packirisamy M, Stiharu I, Darveau A. Hybrid Integrated Silicon Microfluidic Platform for Fluorescence Based Biodetection. Sensors. 2007; 7(9):1901-1915.

Chicago/Turabian Style

Chandrasekaran, Arvind; Acharya, Ashwin; You, Jian L.; Soo, Kim Y.; Packirisamy, Muthukumaran; Stiharu, Ion; Darveau, André. 2007. "Hybrid Integrated Silicon Microfluidic Platform for Fluorescence Based Biodetection." Sensors 7, no. 9: 1901-1915.

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