Abstract: Despite very intensive research in the synthesising of new cytostatics, cisplatin isstill one of the most commonly used anticancer drugs. Therefore, an investigation ofinteractions of cisplatin with different biologically important amino acids, peptides andproteins is very topical. In the present paper, we utilized flow injection analysis coupledwith electrochemical detection to study and characterize the behaviour of various forms ofglutathione (reduced glutathione – GSH, oxidized glutathione – GSSG and S-nitrosoglutathione – GSNO). The optimized conditions were as follows: mobile phase consistedof acetate buffer (pH 3) with a flow rate of 1 mL min-1. Based on results obtained we chose850 mV as the optimal potential for detection of GSH and 1,100 mV as the optimalpotential for detection of GSSG and GSNO. The detection limits of GSH, GSSG andGSNO were 100 pg mL-1, 50 ng mL-1 and 300 pg mL-1, respectively. Further, the optimized technique was used for investigation of interactions between cisplatin and GSH. We were able to observe the interaction between GSH and cisplatin via decrease in the signal corresponding to glutathione. Moreover, we evaluated the formation of the complex by spectrometry. The spectrometric results obtained were in good agreement with electrochemical ones.
Keywords: Glutathione; Carbon paste electrode; Thiols; Cisplatin; Cancer; Flow injection analysis with electrochemical detection.
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Zitka, O.; Huska, D.; Krizkova, S.; Adam, V.; Chavis, G.J.; Trnkova, L.; Horna, A.; Hubalek, J.; Kizek, R. An Investigation of Glutathione-Platinum(II) Interactions by Means of the Flow Injection Analysis Using Glassy Carbon Electrode. Sensors 2007, 7, 1256-1270.
Zitka O, Huska D, Krizkova S, Adam V, Chavis GJ, Trnkova L, Horna A, Hubalek J, Kizek R. An Investigation of Glutathione-Platinum(II) Interactions by Means of the Flow Injection Analysis Using Glassy Carbon Electrode. Sensors. 2007; 7(7):1256-1270.
Zitka, Ondrej; Huska, Dalibor; Krizkova, Sona; Adam, Vojtech; Chavis, Grace J.; Trnkova, Libuse; Horna, Ales; Hubalek, Jaromir; Kizek, Rene. 2007. "An Investigation of Glutathione-Platinum(II) Interactions by Means of the Flow Injection Analysis Using Glassy Carbon Electrode." Sensors 7, no. 7: 1256-1270.