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Sensors 2007, 7(7), 1123-1140; doi:10.3390/s7071123

Indium Tin Oxide-Polyaniline Biosensor: Fabrication and Characterization

Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA
Large Animal Clinical Sciences, Michigan State University, East Lansing, MI 48824, USA
Author to whom correspondence should be addressed.
Received: 6 December 2006 / Accepted: 7 June 2007 / Published: 9 July 2007
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In this study, a novel indium tin oxide (ITO)-polyaniline (Pani) biosensor wasdesigned, fabricated, and characterized. Initial testing was conducted for the detection ofbovine viral diarrhea virus (BVDV). The biosensor design was based upon the specific natureof antibodies to capture the target virus, and the conductive properties of self-doped Pani totranslate the antibody-antigen binding into a quantifying signal. The first part of the study wasto assess the feasibility of the self-doped Pani to be incorporated into the biosensor design byevaluating its several parameters, such as conductivity, physical structure, thermogravimetricproperties, and antibody-binding properties. The second part of the paper highlights thefabrication of the ITO-Pani biosensor to detect the presence of bovine viral diarrhea virus(BVDV) in pure culture. Although only BVDV culture was tested in this study, the biosensoris versatile for the detection of other pathogen of interest by changing the specificity of theantibodies.
Keywords: self-doped polyaniline; biosensor; immunosensor; and pathogen detection self-doped polyaniline; biosensor; immunosensor; and pathogen detection
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Tahir, Z.M.; Alocilja, E.C.; Grooms, D.L. Indium Tin Oxide-Polyaniline Biosensor: Fabrication and Characterization. Sensors 2007, 7, 1123-1140.

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