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Investigation on Clarified Fruit Juice Composition by Using Visible Light Micro-Raman Spectroscopy
Consiglio Nazionale delle Ricerche, Istituto di Cibernetica “E. Caianiello”, Pozzuoli, Italy
Dipartimento di Scienze Fisiche, Università “Federico II”, Naples, Italy
Consorzio Interuniversitario INBB, Sezione di Napoli, Italy
Biophysics and Nanoscience Centre, CNISM, Università della Tuscia, Viterbo, Italy
Dipartimento di Medicina Sperimentale, Seconda Università di Napoli, Naples, Italy
* Author to whom correspondence should be addressed.
Received: 21 September 2007; Accepted: 1 October 2007 / Published: 3 October 2007
Abstract: Liquid samples of clarified apple and apricot juices at different productionstages were investigated using visible light micro-Raman spectroscopy in order to assessits potential in monitoring fruit juice production. As is well-known, pectin plays a strategicrole in the production of clarified juice and the possibility of using Raman for its detectionduring production was therefore evaluated. The data analysis has enabled the clearidentification of pectin. In particular, Raman spectra of apple juice samples from washedand crushed fruits revealed a peak at 845 cm-1 (typical of pectin) which disappears in theRaman spectra of depectinised samples. The fructose content was also revealed by thepresence of four peaks at 823 cm-1, 872 cm-1, 918 cm-1 and 975 cm-1. In the case of apricotjuice, several Raman fingerprints of β-carotene at 1008, 1159 and 1520 cm-1 were alsohighlighted. Present results resulted interesting for the exclusive use of optical methods forthe quantitative determination of the above-mentioned substances in place of thebiochemical assays generally used for this purpose, which are time consuming and requiredifferent chemical reagents for each of them.
Keywords: Fruit juice; Micro-Raman Spectroscopy; Pectin; Pectinase; Apple; Apricot
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Camerlingo, C.; Zenone, F.; Delfino, I.; Diano, N.; Mita, D.G.; Lepore, M. Investigation on Clarified Fruit Juice Composition by Using Visible Light Micro-Raman Spectroscopy. Sensors 2007, 7, 2049-2061.
Camerlingo C, Zenone F, Delfino I, Diano N, Mita DG, Lepore M. Investigation on Clarified Fruit Juice Composition by Using Visible Light Micro-Raman Spectroscopy. Sensors. 2007; 7(10):2049-2061.
Camerlingo, Carlo; Zenone, Flora; Delfino, Ines; Diano, Nadia; Mita, Damiano G.; Lepore, Maria. 2007. "Investigation on Clarified Fruit Juice Composition by Using Visible Light Micro-Raman Spectroscopy." Sensors 7, no. 10: 2049-2061.