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An Optical Biosensor based on Immobilization of Laccase and MBTH in Stacked Films for the Detection of Catechol.
Environmental and Bioprocess Technology Centre, SIRIM Berhad, No.1, Persiaran Dato’ Menteri, Section 2, P.O. Box 7035, 40911 Shah Alam, Selangor D.E., Malaysia
School of Chemical Sciences and Food Technology, Faculty of Sciences and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor D.E., Malaysia
* Author to whom correspondence should be addressed.
Received: 14 March 2007; Accepted: 6 June 2007 / Published: 11 October 2007
Abstract: The fabrication of an optical biosensor by using stacked films where 3-methyl-2-benzothiazolinone hydrazone (MBTH) was immobilized in a hybrid nafion/sol-gelsilicate film and laccase in a chitosan film for the detection of phenolic compounds wasdescribed. Quinone and/or phenoxy radical product from the enzymatic oxidation ofphenolic compounds was allowed to couple with MBTH to form a colored azo-dye productfor spectrophometric detection. The biosensor demonstrated a linear response to catecholconcentration range of 0.5-8.0 mM with detection limit of 0.33 mM and response time of10 min. The reproducibility of the fabricated biosensor was good with RSD value of 5.3 %(n = 8) and stable for at least 2 months. The use of the hybrid materials of nafion/sol-gelsilicate to immobilize laccase has altered the selectivity of the enzyme to various phenoliccompounds such as catechol, guaicol, o-cresol and m-cresol when compared to the non-immobilized enzyme. When immobilized in this hybrid film, the biosensor response onlyto catechol and not other phenolic compounds investigated. Immobilization in this hybridmaterial has enable the biosensor to be more selective to catechol compared with the non-immobilized enzyme. This shows that by a careful selection of different immobilizationmatrices, the selectivity of an enzyme can be modified to yield a biosensor with goodselectivity towards certain targeted analytes.
Keywords: Biosensor; laccase; MBTH; phenolic compounds
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MDPI and ACS Style
Abdullah, J.; Ahmad, M.; Heng, L.Y.; Karuppiah, N.; Sidek, H. An Optical Biosensor based on Immobilization of Laccase and MBTH in Stacked Films for the Detection of Catechol.. Sensors 2007, 7, 2238-2250.
Abdullah J, Ahmad M, Heng LY, Karuppiah N, Sidek H. An Optical Biosensor based on Immobilization of Laccase and MBTH in Stacked Films for the Detection of Catechol.. Sensors. 2007; 7(10):2238-2250.
Abdullah, Jaafar; Ahmad, Musa; Heng, Lee Y.; Karuppiah, Nadarajah; Sidek, Hamidah. 2007. "An Optical Biosensor based on Immobilization of Laccase and MBTH in Stacked Films for the Detection of Catechol.." Sensors 7, no. 10: 2238-2250.