This article is
- freely available
Immobilization of DNA at Glassy Ccarbon Electrodes: A Critical Study of Adsorbed Layer
INFIQC. Departamento de Físico Química. Facultad de Ciencias Químicas. Universidad Nacional de Córdoba. Ciudad Universitaria. 5000 Córdoba. Argentina
* Author to whom correspondence should be addressed.
Received: 23 February 2005 / Accepted: 16 March 2005 / Published: 14 November 2005
Abstract: In this work we present a critical study of the nucleic acid layer immobilized atglassy carbon electrodes. Different studies were performed in order to assess the nature of theinteraction between DNA and the electrode surface. The adsorption and electrooxidation of DNAdemonstrated to be highly dependent on the surface and nature of the glassy carbon electrode. TheDNA layer immobilized at a freshly polished glassy carbon electrode was very stable even afterapplying highly negative potentials. The electron transfer of potassium ferricyanide, catechol anddopamine at glassy carbon surfaces modified with thin (obtained by adsorption under controlledpotential conditions) and thick (obtained by casting the glassy carbon surface with highly concentratedDNA solutions) DNA layers was slower than that at the bare glassy carbon electrode, although thiseffect was dependent on the thickness of the layer and was not charge selective. Raman experimentsshowed an important decrease of the vibrational modes assigned to the nucleobases residues,suggesting a strong interaction of these residues with the electrode surface. The hybridization ofoligo(dG)21 and oligo(dC)21 was evaluated from the guanine oxidation signal and the reduction of theredox indicator Co(phen)33+ . In both cases the chronopotentiometric response indicated that thecompromise of the bases in the interaction of DNA with the electrode surface is too strong, preventingfurther hybridization. In summary, glassy carbon is a useful electrode material to detect DNA in adirect and very sensitive way, but not to be used for the preparation of biorecognition layers by directadsorption of the probe sequence on the electrode surface for detecting the hybridization event.
Keywords: DNA-biosensors; DNA adsorption; glassy carbon electrode; DNA-modified electrode; redox indicator
Article StatisticsClick here to load and display the download statistics.
Notes: Multiple requests from the same IP address are counted as one view.
Cite This Article
MDPI and ACS Style
Pedano, M.L.; Rivas, G.A. Immobilization of DNA at Glassy Ccarbon Electrodes: A Critical Study of Adsorbed Layer. Sensors 2005, 5, 424-447.
Pedano ML, Rivas GA. Immobilization of DNA at Glassy Ccarbon Electrodes: A Critical Study of Adsorbed Layer. Sensors. 2005; 5(6):424-447.
Pedano, M. L.; Rivas, G. A. 2005. "Immobilization of DNA at Glassy Ccarbon Electrodes: A Critical Study of Adsorbed Layer." Sensors 5, no. 6: 424-447.