Sensors 2005, 5(6), 448-464; doi:10.3390/s5060448
Article

Elimination Voltammetry with Linear Scan as a New Detection Method for DNA Sensors

Received: 23 February 2005; Accepted: 4 April 2005 / Published: 14 November 2005
(This article belongs to the Special Issue DNA Based Sensors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The paper describes successful coupling of adsorptive transfer stripping (AdTS) andelimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine (C)and adenine (A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers)were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing ofthe ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through theelimination function conserving the diffusion current component and eliminating kinetic and chargingcurrent components. This function, which provides the elimination signal in a peak-counterpeak form,increased the current sensitivity for A and C resolution, and for the recognition of bases sequences inODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scanrate were found. The combination of EVLS with AdTS procedure can be considered as a newdetection method in a DNA sensor.
Keywords: Oligodeoxynucleotide; DNA biosensor; linear sweep voltammetry; elimination voltammetry with linear scan; reduction of adenine and cytosine; mercury electrode; sequence of nucleotides
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MDPI and ACS Style

Trnkova, L.; Jelen, F.; Petrlova, J.; Adam, V.; Potesil, D.; Kizek, R. Elimination Voltammetry with Linear Scan as a New Detection Method for DNA Sensors. Sensors 2005, 5, 448-464.

AMA Style

Trnkova L, Jelen F, Petrlova J, Adam V, Potesil D, Kizek R. Elimination Voltammetry with Linear Scan as a New Detection Method for DNA Sensors. Sensors. 2005; 5(6):448-464.

Chicago/Turabian Style

Trnkova, Libuse; Jelen, Frantisek; Petrlova, Jitka; Adam, Vojtech; Potesil, David; Kizek, Rene. 2005. "Elimination Voltammetry with Linear Scan as a New Detection Method for DNA Sensors." Sensors 5, no. 6: 448-464.

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