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Elimination Voltammetry with Linear Scan as a New Detection Method for DNA Sensors
Department of Theoretical and Physical Chemistry, Masaryk University, Faculty of Science, Kotlarska 2, 611 37 Brno, Czech Republic
Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, 612 65 Brno, Czech Republic
Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno, Czech Republic
* Author to whom correspondence should be addressed.
Received: 23 February 2005; Accepted: 4 April 2005 / Published: 14 November 2005
Abstract: The paper describes successful coupling of adsorptive transfer stripping (AdTS) andelimination voltammetry with linear scan (EVLS) for the resolution of reduction signals of cytosine (C)and adenine (A) residues in hetero-oligodeoxynucleotides (ODNs). Short ODNs (9-mers and 20-mers)were adsorbed from a small volume on a hanging mercury drop electrode (HMDE). After washing ofthe ODN-modified electrode by water and its transferring to an electrochemical cell, voltammetric curves were measured. The AdTS EVLS was able to determine of C/A ratio of ODNs through theelimination function conserving the diffusion current component and eliminating kinetic and chargingcurrent components. This function, which provides the elimination signal in a peak-counterpeak form,increased the current sensitivity for A and C resolution, and for the recognition of bases sequences inODN chains. Optimal conditions of elimination experiments such as pH, time of adsorption, and scanrate were found. The combination of EVLS with AdTS procedure can be considered as a newdetection method in a DNA sensor.
Keywords: Oligodeoxynucleotide; DNA biosensor; linear sweep voltammetry; elimination voltammetry with linear scan; reduction of adenine and cytosine; mercury electrode; sequence of nucleotides
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Trnkova, L.; Jelen, F.; Petrlova, J.; Adam, V.; Potesil, D.; Kizek, R. Elimination Voltammetry with Linear Scan as a New Detection Method for DNA Sensors. Sensors 2005, 5, 448-464.
Trnkova L, Jelen F, Petrlova J, Adam V, Potesil D, Kizek R. Elimination Voltammetry with Linear Scan as a New Detection Method for DNA Sensors. Sensors. 2005; 5(6):448-464.
Trnkova, Libuse; Jelen, Frantisek; Petrlova, Jitka; Adam, Vojtech; Potesil, David; Kizek, Rene. 2005. "Elimination Voltammetry with Linear Scan as a New Detection Method for DNA Sensors." Sensors 5, no. 6: 448-464.