Sensors 2005, 5(4), 220-234; doi:10.3390/s5040220
Article

Direct Electrochemistry of Redox Proteins and Enzymes Promoted by Carbon Nanotubes

, ,  and * email
Received: 5 June 2004; Accepted: 11 September 2004 / Published: 27 April 2005
(This article belongs to the Special Issue Papers presented at I3S2004, Nanjing)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The redox protein and enzyme, such as hemoglobin (Hb), horseradish peroxidase(HRP) and glucose oxidase (GOx), was immobilized on the surface of the carbon nanotubemodified glassy carbon (CNT/GC) electrode, respectively. The cyclic voltammetric resultsindicated that the redox protein and enzyme underwent effective and stable direct electrontransfer reaction with a pair of nearly symmetrical redox peaks. The formal redox potential,E0’, was almost independent on the scan rates, the average value of E0’ for Hb, HRP andGOx was –0.343 ± 0.001, –0.319 ± 0.002 and –0.456 ± 0.0008 V (vs. SCE,pH 6.9),respectively. The dependence of E0’ on the pH solution indicated that the direct electrontransfer of Hb and HRP was a one-electron-transfer reaction process coupled with oneproton-transfer, while the GOx was a two-electron-transfer coupled with two-protontransfer.The apparent heterogeneous electron transfer rate constant (ks) was 1.25 ± 0.25,2.07 ± 0.69 and 1.74 ± 0.42 s-1 for Hb, HRP and GOx, respectively. The method presentedhere can be easily extended to immobilize other redox enzymes or proteins and obtain theirdirect electrochemistry.
Keywords: carbon nanotube; direct electrochemistry; hemoglobin; horseradish peroxidase; glucose oxidase
PDF Full-text Download PDF Full-Text [395 KB, uploaded 20 June 2014 23:37 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Yin, Y.; Lü, Y.; Wu, P.; Cai, C. Direct Electrochemistry of Redox Proteins and Enzymes Promoted by Carbon Nanotubes. Sensors 2005, 5, 220-234.

AMA Style

Yin Y, Lü Y, Wu P, Cai C. Direct Electrochemistry of Redox Proteins and Enzymes Promoted by Carbon Nanotubes. Sensors. 2005; 5(4):220-234.

Chicago/Turabian Style

Yin, Yajing; Lü, Yafen; Wu, Ping; Cai, Chenxin. 2005. "Direct Electrochemistry of Redox Proteins and Enzymes Promoted by Carbon Nanotubes." Sensors 5, no. 4: 220-234.


Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert