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Sensors 2005, 5(4), 220-234; doi:10.3390/s5040220
Article

Direct Electrochemistry of Redox Proteins and Enzymes Promoted by Carbon Nanotubes

, ,  and *
Received: 5 June 2004 / Accepted: 11 September 2004 / Published: 27 April 2005
(This article belongs to the Special Issue Papers presented at I3S2004, Nanjing)
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Abstract

The redox protein and enzyme, such as hemoglobin (Hb), horseradish peroxidase(HRP) and glucose oxidase (GOx), was immobilized on the surface of the carbon nanotubemodified glassy carbon (CNT/GC) electrode, respectively. The cyclic voltammetric resultsindicated that the redox protein and enzyme underwent effective and stable direct electrontransfer reaction with a pair of nearly symmetrical redox peaks. The formal redox potential,E0’, was almost independent on the scan rates, the average value of E0’ for Hb, HRP andGOx was –0.343 ± 0.001, –0.319 ± 0.002 and –0.456 ± 0.0008 V (vs. SCE,pH 6.9),respectively. The dependence of E0’ on the pH solution indicated that the direct electrontransfer of Hb and HRP was a one-electron-transfer reaction process coupled with oneproton-transfer, while the GOx was a two-electron-transfer coupled with two-protontransfer.The apparent heterogeneous electron transfer rate constant (ks) was 1.25 ± 0.25,2.07 ± 0.69 and 1.74 ± 0.42 s-1 for Hb, HRP and GOx, respectively. The method presentedhere can be easily extended to immobilize other redox enzymes or proteins and obtain theirdirect electrochemistry.
Keywords: carbon nanotube; direct electrochemistry; hemoglobin; horseradish peroxidase; glucose oxidase carbon nanotube; direct electrochemistry; hemoglobin; horseradish peroxidase; glucose oxidase
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Yin, Y.; Lü, Y.; Wu, P.; Cai, C. Direct Electrochemistry of Redox Proteins and Enzymes Promoted by Carbon Nanotubes. Sensors 2005, 5, 220-234.

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