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Sensors 2005, 5(4), 235-244; doi:10.3390/s5040235
Article

Electrochemical Studies of the Inhibition and Activation Effects of Al (III) on the Activity of Bovine Liver Glutamate Dehydrogenase

1,* , 1 and 2
1 College of Chemistry and Environment Science, Department of Environment Science, Nanjing Normal University, Nanjing, 210097, P. R. China 2 State Key Laboratory of Reuse and Control of Pollution, College of Environment Science, Nanjing University, Nanjing, 210093, P. R. China
* Author to whom correspondence should be addressed.
Received: 3 June 2004 / Accepted: 18 November 2004 / Published: 27 April 2005
(This article belongs to the Special Issue Papers presented at I3S2004, Nanjing)
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Abstract

Since the study of Al3+ ion on the enzyme activity by using of electrochemical techniques was rarely found in available literatures, the differential-pulse polarography (DPP) technique was applied to study the effects of Al3+ ion on the glutamate dehydrogenase (GDH) activity in the catalytical reaction of α-KG +NADH+NH4+ ⇔ L-Glu+NAD++H2O by monitoring the DPP reduction current of NAD+. At the plant and animal physiologically relevant pH values (pH=6.5 and 7.5), the GDH enzyme activities were strongly depended on the concentrations of the metal ion in the assay mixture solutions. In the lower Al (III) concentration solutions (<30 μM), the inhibitory effects were shown, which are in accordance with the recently biological findings. With the increase of Al (III) concentrations (30~80 μM), the enzyme GDH activities were activated. However, once the concentration of Al (III) arrived to near 0.1 mM level (>80μM), the inhibition effects of Al (III) were shown again. The cyclic voltammetry of NAD+ and NAD+-GDH in the presence of Al (III) can help to explain some biological phenomena. According to the differential-pulse polarography and cyclic voltammetry experiments, the present research confirmed that the electrochemical technique is a convenient and reliable sensor for accurate determination of enzyme activity in biological and environmental samples.
Keywords: aluminum (III); electrochemical studies; glutamate dehydrogenase activities; inhibition and activation effects aluminum (III); electrochemical studies; glutamate dehydrogenase activities; inhibition and activation effects
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Yang, X.; Li, L.; Bi, S. Electrochemical Studies of the Inhibition and Activation Effects of Al (III) on the Activity of Bovine Liver Glutamate Dehydrogenase. Sensors 2005, 5, 235-244.

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