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Sensors 2005, 5(4), 235-244; doi:10.3390/s5040235
Article

Electrochemical Studies of the Inhibition and Activation Effects of Al (III) on the Activity of Bovine Liver Glutamate Dehydrogenase

1,* , 1 and 2
Received: 3 June 2004; Accepted: 18 November 2004 / Published: 27 April 2005
(This article belongs to the Special Issue Papers presented at I3S2004, Nanjing)
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Abstract: Since the study of Al3+ ion on the enzyme activity by using of electrochemical techniques was rarely found in available literatures, the differential-pulse polarography (DPP) technique was applied to study the effects of Al3+ ion on the glutamate dehydrogenase (GDH) activity in the catalytical reaction of α-KG +NADH+NH4+ ⇔ L-Glu+NAD++H2O by monitoring the DPP reduction current of NAD+. At the plant and animal physiologically relevant pH values (pH=6.5 and 7.5), the GDH enzyme activities were strongly depended on the concentrations of the metal ion in the assay mixture solutions. In the lower Al (III) concentration solutions (<30 μM), the inhibitory effects were shown, which are in accordance with the recently biological findings. With the increase of Al (III) concentrations (30~80 μM), the enzyme GDH activities were activated. However, once the concentration of Al (III) arrived to near 0.1 mM level (>80μM), the inhibition effects of Al (III) were shown again. The cyclic voltammetry of NAD+ and NAD+-GDH in the presence of Al (III) can help to explain some biological phenomena. According to the differential-pulse polarography and cyclic voltammetry experiments, the present research confirmed that the electrochemical technique is a convenient and reliable sensor for accurate determination of enzyme activity in biological and environmental samples.
Keywords: aluminum (III); electrochemical studies; glutamate dehydrogenase activities; inhibition and activation effects aluminum (III); electrochemical studies; glutamate dehydrogenase activities; inhibition and activation effects
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Yang, X.; Li, L.; Bi, S. Electrochemical Studies of the Inhibition and Activation Effects of Al (III) on the Activity of Bovine Liver Glutamate Dehydrogenase. Sensors 2005, 5, 235-244.

AMA Style

Yang X, Li L, Bi S. Electrochemical Studies of the Inhibition and Activation Effects of Al (III) on the Activity of Bovine Liver Glutamate Dehydrogenase. Sensors. 2005; 5(4):235-244.

Chicago/Turabian Style

Yang, Xiaodi; Li, Laifa; Bi, Shuping. 2005. "Electrochemical Studies of the Inhibition and Activation Effects of Al (III) on the Activity of Bovine Liver Glutamate Dehydrogenase." Sensors 5, no. 4: 235-244.



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