Next Article in Journal
Various Structures Based on Nickel Oxide Thick Films as Gamma Radiation Sensors
Previous Article in Journal
Comparison of Urea Determination in Biological Samples by EnFETs Based on pH and pNH4 Detection
Article Menu

Article Versions

Export Article

Open AccessArticle
Sensors 2003, 3(6), 166-175; doi:10.3390/s30600166

A New Laccase Biosensor For Polyphenols Determination

CECUL -Faculdade de Ciências da Universidade de Lisboa, Campo Grande, C 8 1749-016 Lisboa, Portugal
Author to whom correspondence should be addressed.
Received: 1 April 2003 / Accepted: 5 May 2003 / Published: 29 June 2003
Download PDF [189 KB, uploaded 20 June 2014]


The relevance of polyphenols in human health is a well known fact. Prompted by that, a very intensive research has been directed to get a method to detect them, wich will improve the current ones. Laccase (p-diphenol:dioxygen oxidoreductase EC is a multi-copper oxidase, wich couples catalytic oxidation of phenolic substrates with four electron reduction of dioxygen to water [1]. A maximum catalytic response in oxigenated electrolyte was observed between 4.5 and 5.5 [2], while for pH > 6.9 the laccase was found to be inactive [3]. We prepared a biosensor with laccase immobilised on a polyether sulphone membrane, at pH 4.5, wich was applied at Universal Sensors base electrode. Reduction of the product of oxidation of several polyphenols, catalysed by laccase, was done at a potential for wich the polyphenol of interest was found to respond. Reduction of catechol was found to occur at a potential of -200mV, wich is often referred to in the literature for polyphenolic biosensors. However other polyphenols did not respond at that potential. It was observed that (+)- catechin produced a very large cathodic current when +100mV were applied to the laccase biosensor, both in aqueous acetate and 12% ethanol acetate buffer, whereas caffeic acid responded at -50mV. Other polyphenols tested were gallic acid, malvidin, quercetin, rutin, trans-resveratrol
Keywords: Biosensor; Laccase; Polyphenol; (+)- catechin; Caffeic acid Biosensor; Laccase; Polyphenol; (+)- catechin; Caffeic acid
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Gomes, S.A.; Rebelo, M.J. A New Laccase Biosensor For Polyphenols Determination. Sensors 2003, 3, 166-175.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top