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Sensors 2018, 18(7), 2181; https://doi.org/10.3390/s18072181

Spectral Imaging Analysis for Ultrasensitive Biomolecular Detection Using Gold-Capped Nanowire Arrays

1,†
,
1,†
,
1
and
1,2,3,*
1
Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan
2
Institute of Biophotonics, National Yang-Ming University, Taipei 11221, Taiwan
3
Institute of Optoelectronic Sciences, National Taiwan Ocean University, Keelung 20224, Taiwan
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 31 May 2018 / Revised: 4 July 2018 / Accepted: 5 July 2018 / Published: 6 July 2018
(This article belongs to the Special Issue Label-free Optical Nanobiosensors)
View Full-Text   |   Download PDF [1711 KB, uploaded 6 July 2018]   |  

Abstract

A spectral integration combined with a threshold method for the analysis of spectral scanning surface plasmon resonance (SPR) images can significantly increase signal recognition at low concentration of antibody solution. The 12-well SPR sensing plates consisted of gold-capped nanowire arrays with 500-nm period, 80-nm linewidth and 50-nm gold thickness which were used for generating multiple SPR images. A threshold method is introduced to eliminate background noises in spectral scanning images. Combining spectral integration and the threshold method, the detection limit of antibody concentration was 1.23 ng/mL. Using multiple-well SPR sensing plates and the proposed analytical method, multiple kinetic responses with spectral and spatial information on different sensing areas can be sensitively measured. View Full-Text
Keywords: surface plasmon resonance; nanostructures; multiple-wells detection; spectral integration; threshold analysis surface plasmon resonance; nanostructures; multiple-wells detection; spectral integration; threshold analysis
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Tai, Y.-H.; Fu, P.-H.; Lee, K.-L.; Wei, P.-K. Spectral Imaging Analysis for Ultrasensitive Biomolecular Detection Using Gold-Capped Nanowire Arrays. Sensors 2018, 18, 2181.

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