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Sensors 2018, 18(1), 207; doi:10.3390/s18010207

Current Technologies of Electrochemical Immunosensors: Perspective on Signal Amplification

Department of Biomedical Laboratory Science, College of Health Science, Eulji University, Seongnam 13135, Korea
Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon City, Gyunggi Do 164-19, Korea
Department of Biomedical Laboratory Science, School of Medicine, Eulji University, Daejeon 34824, Korea
Department of Medical IT Marketing, College of Health Industry, Eulji University, Seongnam 13135, Korea
Department of Food and Nutrition, Eulji University, Seongnam 13135, Korea
Fermentation Science Program, School of Agribusiness and Agriscience, College of Basic and Applied Sciences, Middle Tennessee State University, Murfreesboro, TN 37132, USA
Korea Research Institute of Standards and Science, P.O. Box 102, Yuseong, Daejon 34113, Korea
Department of Agricultural and Biological Engineering, Bindley Bioscience Center, Purdue Center for Cancer Research, Purdue University, 225 South University Street, West Lafayette, IN 47907, USA
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 8 November 2017 / Revised: 4 January 2018 / Accepted: 6 January 2018 / Published: 12 January 2018
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [1304 KB, uploaded 12 January 2018]   |  


An electrochemical immunosensor employs antibodies as capture and detection means to produce electrical charges for the quantitative analysis of target molecules. This sensor type can be utilized as a miniaturized device for the detection of point-of-care testing (POCT). Achieving high-performance analysis regarding sensitivity has been one of the key issues with developing this type of biosensor system. Many modern nanotechnology efforts allowed for the development of innovative electrochemical biosensors with high sensitivity by employing various nanomaterials that facilitate the electron transfer and carrying capacity of signal tracers in combination with surface modification and bioconjugation techniques. In this review, we introduce novel nanomaterials (e.g., carbon nanotube, graphene, indium tin oxide, nanowire and metallic nanoparticles) in order to construct a high-performance electrode. Also, we describe how to increase the number of signal tracers by employing nanomaterials as carriers and making the polymeric enzyme complex associated with redox cycling for signal amplification. The pros and cons of each method are considered throughout this review. We expect that these reviewed strategies for signal enhancement will be applied to the next versions of lateral-flow paper chromatography and microfluidic immunosensor, which are considered the most practical POCT biosensor platforms. View Full-Text
Keywords: electrochemical immunosensor; nanomaterials; point-of-care testing; signal amplification; electrode scaffold; labeling techniques electrochemical immunosensor; nanomaterials; point-of-care testing; signal amplification; electrode scaffold; labeling techniques

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Cho, I.-H.; Lee, J.; Kim, J.; Kang, M.-S.; Paik, J.K.; Ku, S.; Cho, H.-M.; Irudayaraj, J.; Kim, D.-H. Current Technologies of Electrochemical Immunosensors: Perspective on Signal Amplification. Sensors 2018, 18, 207.

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