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Sensors 2018, 18(1), 131; https://doi.org/10.3390/s18010131

Genotyping of KRAS Mutational Status by the In-Check Lab-on-Chip Platform

1
Institute of Neurological Sciences, Italian National Research Council, Via Paolo Gaifami 18, 95126 Catania, Italy
2
STMicroelectronics, Stradale Primosole 50, 95121 Catania, Italy
3
Analog, MEMS & Sensor Group—HealthCare Business Development Unit, STMicroelectronics, Stradale Primosole 50, 95121 Catania, Italy
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 9 November 2017 / Revised: 14 December 2017 / Accepted: 31 December 2017 / Published: 5 January 2018
(This article belongs to the Special Issue Sensors for Health Monitoring and Disease Diagnosis)
View Full-Text   |   Download PDF [3154 KB, uploaded 5 January 2018]   |  

Abstract

The KRAS oncogene is involved in the pathogenesis of several types of cancer, particularly colorectal cancer (CRC). The most frequent mutations in this gene are associated with poor survival, increased tumor aggressiveness and resistance to therapy with anti-epidermal growth factor receptor (EGFR) antibodies. For this reason, KRAS mutation testing has become increasingly common in clinical practice for personalized cancer treatments of CRC patients. Detection methods for KRAS mutations are currently expensive, laborious, time-consuming and often lack of diagnostic sensitivity and specificity. In this study, we describe the development of a Lab-on-Chip assay for genotyping of KRAS mutational status. This assay, based on the In-Check platform, integrates microfluidic handling, a multiplex polymerase chain reaction (PCR) and a low-density microarray. This integrated sample-to-result system enables the detection of KRAS point mutations, including those occurring in codons 12 and 13 of exon 2, 59 and 61 of exon 3, 117 and 146 of exon 4. Thanks to its miniaturization, automation, rapid analysis, minimal risk of sample contamination, increased accuracy and reproducibility of results, this Lab-on-Chip platform may offer immediate opportunities to simplify KRAS genotyping into clinical routine. View Full-Text
Keywords: Lab-on-Chip; PCR; Microarray; nucleic acids analysis; KRAS; diagnostic Lab-on-Chip; PCR; Microarray; nucleic acids analysis; KRAS; diagnostic
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Guarnaccia, M.; Iemmolo, R.; San Biagio, F.; Alessi, E.; Cavallaro, S. Genotyping of KRAS Mutational Status by the In-Check Lab-on-Chip Platform. Sensors 2018, 18, 131.

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