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Sensors 2017, 17(4), 906; doi:10.3390/s17040906

Characterization of N-Acyl Homoserine Lactones in Vibrio tasmaniensis LGP32 by a Biosensor-Based UHPLC-HRMS/MS Method

1
Sorbonne Universités, UPMC Univ Paris 6, CNRS, Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM), Observatoire Océanologique, F-66650 Banyuls/Mer, France
2
Sorbonne Universités, UPMC Univ Paris 06, CNRS, Observatoire Océanologique de Banyuls (OOB), F-66650 Banyuls/Mer, France
*
Author to whom correspondence should be addressed.
Academic Editors: Jean-Louis Marty, Silvana Andreescu and Akhtar Hayat
Received: 4 April 2017 / Revised: 14 April 2017 / Accepted: 17 April 2017 / Published: 20 April 2017
(This article belongs to the Special Issue Sensors for Toxic and Pathogen Detection)
View Full-Text   |   Download PDF [282 KB, uploaded 20 April 2017]

Abstract

Since the discovery of quorum sensing (QS) in the 1970s, many studies have demonstrated that Vibrio species coordinate activities such as biofilm formation, virulence, pathogenesis, and bioluminescence, through a large group of molecules called N-acyl homoserine lactones (AHLs). However, despite the extensive knowledge on the involved molecules and the biological processes controlled by QS in a few selected Vibrio strains, less is known about the overall diversity of AHLs produced by a broader range of environmental strains. To investigate the prevalence of QS capability of Vibrio environmental strains we analyzed 87 Vibrio spp. strains from the Banyuls Bacterial Culture Collection (WDCM911) for their ability to produce AHLs. This screening was based on three biosensors, which cover a large spectrum of AHLs, and revealed that only 9% of the screened isolates produced AHLs in the defined experimental conditions. Among these AHL-producing strains, Vibrio tasmaniensis LGP32 is a well-known pathogen of bivalves. We further analyzed the diversity of AHLs produced by this strain using a sensitive bioguided UHPLC-HRMS/MS approach (Ultra-High-Performance Liquid Chromatography followed by High-Resolution tandem Mass Spectrometry) and we identified C10-HSL, OH-C12-HSL, oxo-C12-HSL and C14:1-HSL as QS molecules. This is the first report that documents the production of AHL by Vibrio tasmaniensis LGP32. View Full-Text
Keywords: quorum sensing; N-acyl-homoserine lactone (AHL); Vibrio tasmaniensis LGP32; fractionation; biosensors; UHPLC-HRMS/MS quorum sensing; N-acyl-homoserine lactone (AHL); Vibrio tasmaniensis LGP32; fractionation; biosensors; UHPLC-HRMS/MS
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Girard, L.; Blanchet, É.; Intertaglia, L.; Baudart, J.; Stien, D.; Suzuki, M.; Lebaron, P.; Lami, R. Characterization of N-Acyl Homoserine Lactones in Vibrio tasmaniensis LGP32 by a Biosensor-Based UHPLC-HRMS/MS Method. Sensors 2017, 17, 906.

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