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Sensors 2017, 17(5), 1025; doi:10.3390/s17051025

Supramolecular Recognition of Escherichia coli Bacteria by Fluorescent Oligo(Phenyleneethynylene)s with Mannopyranoside Termini Groups

1
Centro de Investigación en Química Aplicada (CIQA), Blvd. Enrique Reyna 140, Saltillo 25294, Mexico
2
Istituto dei Sistemi Complessi Via Madonna del Piano 10, Sesto Fiorentino 50019, Italy
*
Authors to whom correspondence should be addressed.
Academic Editors: Jean Lousi Marty, Silvana Andreescu and Akhtar Hayat
Received: 24 January 2017 / Revised: 15 March 2017 / Accepted: 11 April 2017 / Published: 4 May 2017
(This article belongs to the Special Issue Sensors for Toxic and Pathogen Detection)
View Full-Text   |   Download PDF [4231 KB, uploaded 4 May 2017]   |  

Abstract

Escherichia coli is one the most common bacteria responsible of uropathogenic diseases, which motives the search for rapid and easy methods of detection. By taking advantage of the specific interactions between mannose and type 1 fimbriae, in this work two fluorescent phenyleneethynylene (PE) trimers bearing one or two 4-aminophenyl-α-D-mannopyranoside termini groups were synthesized for the detection of E. coli. Three bacterial strains: ORN 178 (fimbriae I expression), ORN 208 (mutant serotype with no fimbriae expression) and one obtained from a local hospital (SS3) were used. Laser Scanning Confocal Microscopy (LSCM) and Surface Plasmon Resonance (SPR) were applied for the interaction studies following two different approaches: (1) mixing the oligomer solutions with the bacterial suspension, which permitted the observation of stained bacteria and by (2) biosensing as thin films, where bacteria adhered on the surface-functionalized substrate. LSCM allows one to easily visualize that two mannose groups are necessary to have a specific interaction with the fimbriae 1. The sensitivity of SPR assays to E. coli was 104 colony forming unit (CFU)/mL at 50 µL/min flow rate. The combination of PE units with two mannose groups results in a novel molecule that can be used as a specific fluorescent marker as well as a transducer for the detection of E. coli. View Full-Text
Keywords: phenyleneethynylenes; Escherichia coli; SPR phenyleneethynylenes; Escherichia coli; SPR
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Arias, E.; Méndez, M.T.; Arias, E.; Moggio, I.; Ledezma, A.; Romero, J.; Margheri, G.; Giorgetti, E. Supramolecular Recognition of Escherichia coli Bacteria by Fluorescent Oligo(Phenyleneethynylene)s with Mannopyranoside Termini Groups. Sensors 2017, 17, 1025.

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