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Sensors 2017, 17(4), 753; doi:10.3390/s17040753

Strategy for Accurate Detection of Escherichia coli O157:H7 in Ground Pork Using a Lateral Flow Immunoassay

1
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
2
Department of Science and Technology, Nanchang University, Nanchang 330047, China
*
Author to whom correspondence should be addressed.
Academic Editors: Jean-Louis Marty, Silvana Andreescu and Akhtar Hayat
Received: 9 February 2017 / Revised: 21 March 2017 / Accepted: 28 March 2017 / Published: 2 April 2017
(This article belongs to the Special Issue Sensors for Toxic and Pathogen Detection)
View Full-Text   |   Download PDF [898 KB, uploaded 14 April 2017]   |  

Abstract

Escherichia coli O157:H7 is known to cause serious diseases including hemorrhagic colitis and hemolytic uremic syndrome. A gold nanoparticle lateral flow immunoassay (Au-LFIA) was used to detect Escherichia coli O157:H7 in ground pork samples. False-positive results were detected using Au-LFIA; a Citrobacter freundii strain was isolated from the ground pork samples and identified by using CHROmagarTM plates, API 20E, and 16S RNA sequencing. Since C. freundii showed cross-reactivity with E. coli O157:H7 when Au-LFIA test strips were used, a novel method combining modified enrichment with a lateral flow immunoassay for accurate and convenient detection of E. coli O157:H7 in ground pork was developed in this study to minimize these false positives. MacConkey broth was optimized for E. coli O157:H7 enrichment and C. freundii inhibition by the addition of 5 mg/L potassium tellurite and 0.10 mg/L cefixime. Using the proposed modified enrichment procedure, the false-positive rate of ground pork samples spiked with 100 CFU/g C. freundii decreased to 5%. View Full-Text
Keywords: enrichment; E. coli O157:H7; ground pork sample; false positive enrichment; E. coli O157:H7; ground pork sample; false positive
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MDPI and ACS Style

Cheng, S.; Chen, M.-H.; Zhang, G.-G.; Yu, Z.-B.; Liu, D.-F.; Xiong, Y.-H.; Wei, H.; Lai, W.-H. Strategy for Accurate Detection of Escherichia coli O157:H7 in Ground Pork Using a Lateral Flow Immunoassay. Sensors 2017, 17, 753.

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