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Sensors 2017, 17(12), 2859; https://doi.org/10.3390/s17122859

A New Laccase Based Biosensor for Tartrazine

1
School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor 43600, Malaysia
2
Polymer Research Center, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor 43600, Malaysia
*
Author to whom correspondence should be addressed.
Received: 4 October 2017 / Revised: 13 November 2017 / Accepted: 30 November 2017 / Published: 9 December 2017
(This article belongs to the Special Issue Development of Enzymatic Electrochemical Biosensors and Applications)
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Abstract

Laccase enzyme, a commonly used enzyme for the construction of biosensors for phenolic compounds was used for the first time to develop a new biosensor for the determination of the azo-dye tartrazine. The electrochemical biosensor was based on the immobilization of laccase on functionalized methacrylate-acrylate microspheres. The biosensor membrane is a composite of the laccase conjugated microspheres and gold nanoparticles (AuNPs) coated on a carbon-paste screen-printed electrode. The reaction involving tartrazine can be catalyzed by laccase enzyme, where the current change was measured by differential pulse voltammetry (DPV) at 1.1 V. The anodic peak current was linear within the tartrazine concentration range of 0.2 to 14 μM (R2 = 0.979) and the detection limit was 0.04 μM. Common food ingredients or additives such as glucose, sucrose, ascorbic acid, phenol and sunset yellow did not interfere with the biosensor response. Furthermore, the biosensor response was stable up to 30 days of storage period at 4 °C. Foods and beverage were used as real samples for the biosensor validation. The biosensor response to tartrazine showed no significant difference with a standard HPLC method for tartrazine analysis. View Full-Text
Keywords: tartrazine; methacrylate-acrylate microspheres; AuNPs; electrochemical sensor; enzymatic biosensor tartrazine; methacrylate-acrylate microspheres; AuNPs; electrochemical sensor; enzymatic biosensor
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Mazlan, S.Z.; Lee, Y.H.; Hanifah, S.A. A New Laccase Based Biosensor for Tartrazine. Sensors 2017, 17, 2859.

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