A New Laccase Based Biosensor for Tartrazine
AbstractLaccase enzyme, a commonly used enzyme for the construction of biosensors for phenolic compounds was used for the first time to develop a new biosensor for the determination of the azo-dye tartrazine. The electrochemical biosensor was based on the immobilization of laccase on functionalized methacrylate-acrylate microspheres. The biosensor membrane is a composite of the laccase conjugated microspheres and gold nanoparticles (AuNPs) coated on a carbon-paste screen-printed electrode. The reaction involving tartrazine can be catalyzed by laccase enzyme, where the current change was measured by differential pulse voltammetry (DPV) at 1.1 V. The anodic peak current was linear within the tartrazine concentration range of 0.2 to 14 μM (R2 = 0.979) and the detection limit was 0.04 μM. Common food ingredients or additives such as glucose, sucrose, ascorbic acid, phenol and sunset yellow did not interfere with the biosensor response. Furthermore, the biosensor response was stable up to 30 days of storage period at 4 °C. Foods and beverage were used as real samples for the biosensor validation. The biosensor response to tartrazine showed no significant difference with a standard HPLC method for tartrazine analysis. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 551 KB)
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Mazlan, S.Z.; Lee, Y.H.; Hanifah, S.A. A New Laccase Based Biosensor for Tartrazine. Sensors 2017, 17, 2859.
Mazlan SZ, Lee YH, Hanifah SA. A New Laccase Based Biosensor for Tartrazine. Sensors. 2017; 17(12):2859.Chicago/Turabian Style
Mazlan, Siti Z.; Lee, Yook H.; Hanifah, Sharina A. 2017. "A New Laccase Based Biosensor for Tartrazine." Sensors 17, no. 12: 2859.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.