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Sensors 2018, 18(1), 101; doi:10.3390/s18010101

Electrochemical Sunset Yellow Biosensor Based on Photocured Polyacrylamide Membrane for Food Dye Monitoring

1
School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia
2
Fuel Cell Institute, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia
3
Polymer Research Center, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia
*
Author to whom correspondence should be addressed.
Received: 4 November 2017 / Revised: 7 December 2017 / Accepted: 15 December 2017 / Published: 1 January 2018
(This article belongs to the Special Issue Development of Enzymatic Electrochemical Biosensors and Applications)
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Abstract

An enzyme-based electrochemical biosensor was investigated for the analysis of Sunset Yellow synthetic food dye. A glassy carbon electrode was coated with a poly(acrylamide-co-ethyl methacrylate) membrane to immobilize laccase using a single-step photopolymerization procedure. Poly(acrylamide-co-ethyl methacrylate) membrane was demonstrated to have acceptable water absorption and suitable for biosensor application. Sunset Yellow biosensor exhibited a linear response range from 0.08 to 10.00 µM with a detection limit of 0.02 µM. This biosensor was successfully used to determine Sunset Yellow in soft drinks with recoveries of 99.0–101.6%. The method was validated using high-performance liquid chromatography, indicating the biosensor can be as a promising alternative method for Sunset Yellow detection. View Full-Text
Keywords: electrochemical biosensor; Sunset Yellow; synthetic food dye; laccase; photopolymerization electrochemical biosensor; Sunset Yellow; synthetic food dye; laccase; photopolymerization
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Rozi, N.; Ahmad, A.; Yook Heng, L.; Shyuan, L.K.; Hanifah, S.A. Electrochemical Sunset Yellow Biosensor Based on Photocured Polyacrylamide Membrane for Food Dye Monitoring. Sensors 2018, 18, 101.

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