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Sensors 2017, 17(11), 2503; https://doi.org/10.3390/s17112503

Impedance-Based Living Cell Analysis for Clinical Diagnosis of Type I Allergy

Department of Dermatology, Graduate School of Biomedical and Health Science, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan
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Received: 30 August 2017 / Revised: 23 October 2017 / Accepted: 27 October 2017 / Published: 31 October 2017
(This article belongs to the Special Issue Sensors for Health Monitoring and Disease Diagnosis)
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Abstract

Non-invasive real time evaluation of living cell conditions and functions are increasingly desired in the field of clinical diagnosis. For diagnosis of type I allergy, the identification of antigens that induces activation of mast cells and basophils is crucial to avoid symptoms of allergic diseases. However, conventional tests, such as detection of antigen-specific IgE antibody and skin tests, are either of low reliability or are invasive. To overcome such problems, we hereby applied an impedance sensor for label-free and real-time monitoring of mast cell reactions in response to stimuli. When IgE-sensitized RBL-2H3 cells cultured on the electrodes were stimulated with various concentrations of antigens, dose-dependent cell index (CI) increases were detected. Moreover, we confirmed that the impedance sensor detected morphological changes rather than degranulation as the indicator of cell activation. Furthermore, the CI of human IgE receptor-expressing cells (RBL-48 cells) treated with serum of a sweat allergy-positive patient, but not with serum from a sweat allergy-negative patient, significantly increased in response to purified human sweat antigen. We thus developed a technique to detect the activation of living cells in response to stimuli without any labeling using the impedance sensor. This system may represent a high reliable tool for the diagnosis of type I allergy. View Full-Text
Keywords: impedance sensor; diagnosis of type I allergy; mast cells; human IgE receptor-expressing cells; IgE antibody; serum; histamine release test impedance sensor; diagnosis of type I allergy; mast cells; human IgE receptor-expressing cells; IgE antibody; serum; histamine release test
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Irifuku, R.; Yanase, Y.; Kawaguchi, T.; Ishii, K.; Takahagi, S.; Hide, M. Impedance-Based Living Cell Analysis for Clinical Diagnosis of Type I Allergy. Sensors 2017, 17, 2503.

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