The Evaluation of a Low-Cost Colorimeter for Glucose Detection in Salivary Samples
AbstractGiven the limited access to healthcare resources, low-income settings require the development of affordable technology. Here we present the design and evaluation of a low-cost colorimeter applied to the non-invasive monitoring of Diabetes Mellitus through the detection of glucose in salival fluid. Samples were processed by the glucose oxidase-peroxidase enzymatic system and analyzed with the development equipment. A light emission diode of 532.5 nm was used as an excitation source and a RGB module was used as a receptor. A calibration curve to quantify the concentration of salivary glucose (0 to 18 mg/dL) was carried out by relating the RGB components registered with glucose concentrations, achieving a limit of detection of 0.17 mg/dL with a CV of 5% (n = 3). Salivary samples of diabetic and healthy volunteers were processed with the equipment showing an average concentration of 1.5519 ± 0.4511 mg/dL for the first and 4.0479 ± 1.6103 mg/dL for the last, allowing a discrimination between both groups. Results were validated against a UV-Vis-NIR spectrophotometer with a correspondence of R2 of 0.98194 between both instruments. Results suggest the potential application of the developed device to the sensitive detection of relevant analytes with a low-cost, user-friendly, low-power and portable instrumentation. View Full-Text
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Dominguez, R.B.; Orozco, M.A.; Chávez, G.; Márquez-Lucero, A. The Evaluation of a Low-Cost Colorimeter for Glucose Detection in Salivary Samples. Sensors 2017, 17, 2495.
Dominguez RB, Orozco MA, Chávez G, Márquez-Lucero A. The Evaluation of a Low-Cost Colorimeter for Glucose Detection in Salivary Samples. Sensors. 2017; 17(11):2495.Chicago/Turabian Style
Dominguez, Rocio B.; Orozco, Miguel A.; Chávez, Giovanny; Márquez-Lucero, Alfredo. 2017. "The Evaluation of a Low-Cost Colorimeter for Glucose Detection in Salivary Samples." Sensors 17, no. 11: 2495.
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