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Sensors 2017, 17(10), 2249; https://doi.org/10.3390/s17102249

Rational Design of Peptide-Functionalized Surface Plasmon Resonance Sensor for Specific Detection of TNT Explosive

1
Research and Development Center for Taste and Odor Sensing, Kyushu University, 744 Motooka, Nishiku, Fukuoka 819-0395, Japan
2
Department of Chemical Science and Engineering, Tokyo Institute of Technology, 2-12-1, O-okayama, Meguro-ku, Tokyo 152-8552, Japan
3
Department of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishiku, Fukuoka 819-0395, Japan
*
Author to whom correspondence should be addressed.
Received: 5 September 2017 / Revised: 25 September 2017 / Accepted: 28 September 2017 / Published: 30 September 2017
(This article belongs to the Special Issue Electronic Tongues and Electronic Noses)
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Abstract

In this study, a rationally-designed 2,4,6-trinitrotoluene (TNT) binding peptide derived from an amino acid sequence of the complementarity-determining region (CDR) of an anti-TNT monoclonal antibody was used for TNT detection based on a maleimide-functionalized surface plasmon resonance (SPR) sensor. By antigen-docking simulation and screening, the TNT binding candidate peptides were obtained as TNTHCDR1 derived from the heavy chain of CDR1, TNTHCDR2 derived from CDR2, and TNTHCDR3 from CDR3 of an anti-TNT antibody. The binding events between candidate peptides and TNT were evaluated using the SPR sensor by direct determination based on the 3-aminopropyltriethoxysilane (APTES) surface. The TNT binding peptide was directly immobilized on the maleimide-functionalized sensor chip surface from N-γ-maleimidobutyryl-oxysuccinimide ester (GMBS). The results demonstrated that peptide TNTHCDR3 was identified and selected as a TNT binding peptide among the other two candidate peptides. Five kinds of TNT analogues were also investigated to testify the selectivity of TNT binding peptide TNTHCDR3. Furthermore, the results indicated that the APTES-GMBS-based SPR sensor chip procedure featured a great potential application for the direct detection of TNT. View Full-Text
Keywords: rationally designed; TNT binding peptides; amino acid sequence; APTES; surface plasmon resonance; N-γ-maleimidobutyryl-oxysuccinimide ester rationally designed; TNT binding peptides; amino acid sequence; APTES; surface plasmon resonance; N-γ-maleimidobutyryl-oxysuccinimide ester
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Wang, J.; Muto, M.; Yatabe, R.; Onodera, T.; Tanaka, M.; Okochi, M.; Toko, K. Rational Design of Peptide-Functionalized Surface Plasmon Resonance Sensor for Specific Detection of TNT Explosive. Sensors 2017, 17, 2249.

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