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Sensors 2017, 17(1), 34; doi:10.3390/s17010034

Organophosphorous Pesticide Detection in Olive Oil by Using a Miniaturized, Easy-to-Use, and Cost-Effective Biosensor Combined with QuEChERS for Sample Clean-Up

1
Department of Chemical Science and Technologies, University of Rome “Tor Vergata”, Via della Ricerca Scientifica, 00133 Rome, Italy
2
Institute of Crystallography (IC-CNR), Via Salaria km 29.300, 00015 Monterotondo, Italy
3
Department of Analytical Chemistry, Institute of Chemical Engineering, Ural Federal University Named After the First President of Russia B. N. Yeltsin, Mira 19, 620002 Yekaterinburg, Russian Federation
*
Author to whom correspondence should be addressed.
Academic Editors: Jesus Iniesta Valcarcel and Craig E. Banks
Received: 4 November 2016 / Revised: 13 December 2016 / Accepted: 20 December 2016 / Published: 24 December 2016
(This article belongs to the Special Issue Recent Advances in Biosensors Based Screen Printed Platforms)
View Full-Text   |   Download PDF [1687 KB, uploaded 24 December 2016]   |  

Abstract

Herein, we report a portable electrochemical biosensor based on butyrylcholinesterase (BChE) immobilized on carbon black (CB)-modified screen-printed electrodes (SPEs) for the detection of organophosphorous pesticides in olive oil. The BChE/CB-SPE biosensor was developed to detect paraoxon in standard solutions as well as in olive oil samples previously treated with the QuEChERS method to extract pesticides from the whole fatty matrix. The biosensor shows a linear concentration range of between 20 and 100 ppb for paraoxon both in standard solutions (phosphate buffer 0.05 M) and in olive oil extracts, with a detection limit of 6 ppb in olive oil extract, corresponding to 10% of inhibition. The accuracy of this biosensor in olive oil samples was assessed with olive oil spiked with paraoxon, obtaining satisfactory recovery values. View Full-Text
Keywords: organophosphorous pesticides; electrochemical biosensor; butyrylcholinesterase; carbon black; screen-printed electrodes; QuEChERS organophosphorous pesticides; electrochemical biosensor; butyrylcholinesterase; carbon black; screen-printed electrodes; QuEChERS
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Arduini, F.; Forchielli, M.; Scognamiglio, V.; Nikolaevna, K.A.; Moscone, D. Organophosphorous Pesticide Detection in Olive Oil by Using a Miniaturized, Easy-to-Use, and Cost-Effective Biosensor Combined with QuEChERS for Sample Clean-Up. Sensors 2017, 17, 34.

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