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Sensors 2016, 16(9), 1342; doi:10.3390/s16091342

Sensitive Bioanalysis Based on in-Situ Droplet Anodic Stripping Voltammetric Detection of CdS Quantum Dots Label after Enhanced Cathodic Preconcentration

1
Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China
2
Liuzhou Traditional Chinese Medicine Hospital, Liuzhou 545001, China
*
Author to whom correspondence should be addressed.
Academic Editor: Huangxian Ju
Received: 17 June 2016 / Revised: 29 July 2016 / Accepted: 4 August 2016 / Published: 23 August 2016
(This article belongs to the Special Issue Nanobiosensing for Sensors)
View Full-Text   |   Download PDF [2522 KB, uploaded 23 August 2016]   |  

Abstract

We report a protocol of CdS-labeled sandwich-type amperometric bioanalysis with high sensitivity, on the basis of simultaneous chemical-dissolution/cathodic-enrichment of the CdS quantum dot biolabel and anodic stripping voltammetry (ASV) detection of Cd directly on the bioelectrode. We added a microliter droplet of 0.1 M aqueous HNO3 to dissolve CdS on the bioelectrode and simultaneously achieved the potentiostatic cathodic preconcentration of Cd by starting the potentiostatic operation before HNO3 addition, which can largely increase the ASV signal. Our protocol was used for immunoanalysis and aptamer-based bioanalysis of several proteins, giving limits of detection of 4.5 fg·mL−1 for human immunoglobulin G, 3.0 fg·mL−1 for human carcinoembryonic antigen (CEA), 4.9 fg·mL−1 for human α-fetoprotein (AFP), and 0.9 fM for thrombin, which are better than many reported results. The simultaneous and sensitive analysis of CEA and AFP at two screen-printed carbon electrodes was also conducted by our protocol. View Full-Text
Keywords: immunoelectrode; aptamer-electrode; screen-printed carbon electrodes; CdS quantum dot-labeled amperometric bioanalysis; signal amplification immunoelectrode; aptamer-electrode; screen-printed carbon electrodes; CdS quantum dot-labeled amperometric bioanalysis; signal amplification
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Qin, X.; Wang, L.; Xie, Q. Sensitive Bioanalysis Based on in-Situ Droplet Anodic Stripping Voltammetric Detection of CdS Quantum Dots Label after Enhanced Cathodic Preconcentration. Sensors 2016, 16, 1342.

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