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Sensors 2016, 16(3), 290; doi:10.3390/s16030290

Fluorescence Characterization of Gold Modified Liposomes with Antisense N-myc DNA Bound to the Magnetisable Particles with Encapsulated Anticancer Drugs (Doxorubicin, Ellipticine and Etoposide)

1
Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
2
Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic
3
Department of Biochemistry, Faculty of Science, Charles University, Albertov 2030, CZ-12840 Prague, Czech Republic
4
Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine, Charles University, and University Hospital, Motol V Uvalu 84, CZ-15006 Prague, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Alexander Star
Received: 16 November 2015 / Revised: 3 February 2016 / Accepted: 18 February 2016 / Published: 25 February 2016
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [3273 KB, uploaded 25 February 2016]   |  

Abstract

Liposome-based drug delivery systems hold great potential for cancer therapy. The aim of this study was to design a nanodevice for targeted anchoring of liposomes (with and without cholesterol) with encapsulated anticancer drugs and antisense N-myc gene oligonucleotide attached to its surface. To meet this main aim, liposomes with encapsulated doxorubicin, ellipticine and etoposide were prepared. They were further characterized by measuring their fluorescence intensity, whereas the encapsulation efficiency was estimated to be 16%. The hybridization process of individual oligonucleotides forming the nanoconstruct was investigated spectrophotometrically and electrochemically. The concentrations of ellipticine, doxorubicin and etoposide attached to the nanoconstruct in gold nanoparticle-modified liposomes were found to be 14, 5 and 2 µg·mL−1, respectively. The study succeeded in demonstrating that liposomes are suitable for the transport of anticancer drugs and the antisense oligonucleotide, which can block the expression of the N-myc gene. View Full-Text
Keywords: liposome; gold nanoparticles; N-myc; doxorubicin; ellipticine; etoposide liposome; gold nanoparticles; N-myc; doxorubicin; ellipticine; etoposide
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Skalickova, S.; Nejdl, L.; Kudr, J.; Ruttkay-Nedecky, B.; Jimenez Jimenez, A.M.; Kopel, P.; Kremplova, M.; Masarik, M.; Stiborova, M.; Eckschlager, T.; Adam, V.; Kizek, R. Fluorescence Characterization of Gold Modified Liposomes with Antisense N-myc DNA Bound to the Magnetisable Particles with Encapsulated Anticancer Drugs (Doxorubicin, Ellipticine and Etoposide). Sensors 2016, 16, 290.

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