Next Article in Journal
An Accurate GPS-IMU/DR Data Fusion Method for Driverless Car Based on a Set of Predictive Models and Grid Constraints
Previous Article in Journal
Registration of Feature-Poor 3D Measurements from Fringe Projection
Article Menu

Export Article

Open AccessArticle
Sensors 2016, 16(3), 282; doi:10.3390/s16030282

Utilisation of Quartz Crystal Microbalance Sensors with Dissipation (QCM-D) for a Clauss Fibrinogen Assay in Comparison with Common Coagulation Reference Methods

Biosensorik-Gruppe, Institut für Klinische und Experimentelle Transfusionsmedizin (IKET), Universitätsklinikum Tübingen, Otfried-Müller-Str. 27, 72076 Tübingen, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Sandeep Kumar Vashist
Received: 7 December 2015 / Revised: 15 February 2016 / Accepted: 18 February 2016 / Published: 24 February 2016
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [4655 KB, uploaded 25 February 2016]   |  

Abstract

The determination of fibrinogen levels is one of the most important coagulation measurements in medicine. It plays a crucial part in diagnostic and therapeutic decisions, often associated with time-critical conditions. The commonly used measurement is the Clauss fibrinogen assay (CFA) where plasma is activated by thrombin reagent and which is conducted by mechanical/turbidimetric devices. As quartz crystal microbalance sensors with dissipation (QCM-D) based devices have a small footprint, can be operated easily and allow measurements independently from sample transportation time, laboratory location, availability and opening hours, they offer a great opportunity to complement laboratory CFA measurements. Therefore, the objective of the work was to (1) transfer the CFA to the QCM-D method; (2) develop an easy, time- and cost-effective procedure and (3) compare the results with references. Different sensor coatings (donor’s own plasma; gold surface) and different QCM-D parameters (frequency signal shift; its calculated turning point; dissipation signal shift) were sampled. The results demonstrate the suitability for a QCM-D-based CFA in physiological fibrinogen ranges. Results were obtained in less than 1 min and in very good agreement with a standardized reference (Merlin coagulometer). The results provide a good basis for further investigation and pave the way to a possible application of QCM-D in clinical and non-clinical routine in the medical field. View Full-Text
Keywords: Quartz Crystal Microbalance with dissipation (QCM-D); Clauss Fibrinogen Assay; Blood Coagulation; Blood Viscoelasticity; Fibrinogen Quartz Crystal Microbalance with dissipation (QCM-D); Clauss Fibrinogen Assay; Blood Coagulation; Blood Viscoelasticity; Fibrinogen
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Oberfrank, S.; Drechsel, H.; Sinn, S.; Northoff, H.; Gehring, F.K. Utilisation of Quartz Crystal Microbalance Sensors with Dissipation (QCM-D) for a Clauss Fibrinogen Assay in Comparison with Common Coagulation Reference Methods. Sensors 2016, 16, 282.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top