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Sensors 2016, 16(2), 232; doi:10.3390/s16020232

Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization

1
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China
2
School of Science, Minzu University of China, Beijing 100081, China
*
Authors to whom correspondence should be addressed.
Academic Editors: Zhiping Wang and Charles Chun Yang
Received: 28 December 2015 / Revised: 21 January 2016 / Accepted: 2 February 2016 / Published: 16 February 2016
(This article belongs to the Special Issue Micro/Nano Fluidic Devices and Bio-MEMS)
View Full-Text   |   Download PDF [3314 KB, uploaded 16 February 2016]   |  

Abstract

This article reviews recent developments in microfluidic systems enabling high-throughput characterization of single-cell proteins. Four key perspectives of microfluidic platforms are included in this review: (1) microfluidic fluorescent flow cytometry; (2) droplet based microfluidic flow cytometry; (3) large-array micro wells (microengraving); and (4) large-array micro chambers (barcode microchips). We examine the advantages and limitations of each technique and discuss future research opportunities by focusing on three key performance parameters (absolute quantification, sensitivity, and throughput). View Full-Text
Keywords: microfluidics; single-cell analysis; protein characterization; high-throughput microfluidics; single-cell analysis; protein characterization; high-throughput
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Fan, B.; Li, X.; Chen, D.; Peng, H.; Wang, J.; Chen, J. Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization. Sensors 2016, 16, 232.

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