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Sensors 2016, 16(12), 2154; doi:10.3390/s16122154

Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen

1
Department of Chemical Engineering, Kangwon National University, Samcheok 25913, Korea
2
Nanobio Application Team, National NanoFab Center (NNFC), Daejeon 34141, Korea
3
Department of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon 22212, Korea
4
Research and Development Center, Tomorrow & Solution (T&S), E19-604, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Korea
5
Research Center 2, Eudipia Inc., Cheongju 361-951, Korea
*
Authors to whom correspondence should be addressed.
Academic Editor: Huangxian Ju
Received: 12 September 2016 / Revised: 30 November 2016 / Accepted: 12 December 2016 / Published: 16 December 2016
(This article belongs to the Special Issue Nanobiosensing for Sensors)
View Full-Text   |   Download PDF [11108 KB, uploaded 21 December 2016]   |  

Abstract

In this study, we developed lateral flow assay (LFA) biosensors for the detection of hepatitis B surface antigens using well-controlled gold nanoparticles (AuNPs). To enhance colorimetric signals, a seeded growth method was used for the preparation of size-controlled AuNPs with a narrow size distribution. Different sizes of AuNPs in the range of 342–137.8 nm were conjugated with antibodies and then optimized for the efficient detection of LFA biosensors. The conjugation stability was investigated by UV-vis spectroscopy of AuNP dispersion at various pH values and concentrations of antibody. Based on optimized conjugation conditions, the use of 42.7 ± 0.8 nm AuNPs exhibited superior performance for the detection of LFAs relative to other sizes of AuNPs. View Full-Text
Keywords: gold; nanoparticle; hepatitis B surface antigen; lateral flow assay; conjugation gold; nanoparticle; hepatitis B surface antigen; lateral flow assay; conjugation
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MDPI and ACS Style

Kim, D.S.; Kim, Y.T.; Hong, S.B.; Kim, J.; Heo, N.S.; Lee, M.-K.; Lee, S.J.; Kim, B.I.; Kim, I.S.; Huh, Y.S.; Choi, B.G. Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen. Sensors 2016, 16, 2154.

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