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Sensors 2016, 16(10), 1684; doi:10.3390/s16101684

ESIPT-Based Photoactivatable Fluorescent Probe for Ratiometric Spatiotemporal Bioimaging

1
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China
2
Environment Monitoring Department, Changsha Environmental Protection College, Changsha 410004, China
*
Author to whom correspondence should be addressed.
Academic Editor: Jong Seung Kim
Received: 24 August 2016 / Revised: 20 September 2016 / Accepted: 22 September 2016 / Published: 12 October 2016
(This article belongs to the Special Issue Colorimetric and Fluorescent Sensor)
View Full-Text   |   Download PDF [980 KB, uploaded 12 October 2016]   |  

Abstract

Photoactivatable fluorophores have become an important technique for the high spatiotemporal resolution of biological imaging. Here, we developed a novel photoactivatable probe (PHBT), which is based on 2-(2-hydroxyphenyl)benzothiazole (HBT), a small organic fluorophore known for its classic luminescence mechanism through excited-state intramolecular proton transfer (ESIPT) with the keto form and the enol form. After photocleavage, PHBT released a ratiometric fluorophore HBT, which showed dual emission bands with more than 73-fold fluorescence enhancement at 512 nm in buffer and more than 69-fold enhancement at 452 nm in bovine serum. The probe displayed a high ratiometric imaging resolution and is believed to have a wide application in biological imaging. View Full-Text
Keywords: ESIPT; photoactivatable; ratiometric ESIPT; photoactivatable; ratiometric
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Zhou, X.; Jiang, Y.; Zhao, X.; Guo, D. ESIPT-Based Photoactivatable Fluorescent Probe for Ratiometric Spatiotemporal Bioimaging. Sensors 2016, 16, 1684.

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