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Sensors 2016, 16(1), 79; doi:10.3390/s16010079

Synthesis and Application of an Aldazine-Based Fluorescence Chemosensor for the Sequential Detection of Cu2+ and Biological Thiols in Aqueous Solution and Living Cells

1
Key Laboratory for Functional Material, Educational Department of Liaoning Province, University of Science and Technology Liaoning, Anshan 114051, China
2
Department of Forensic Medicine, Xinxiang Medical University, Xinxiang, He‘nan 453003, China
3
Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW 2109, Australia
*
Authors to whom correspondence should be addressed.
Academic Editor: W. Rudolf Seitz
Received: 20 November 2015 / Revised: 28 December 2015 / Accepted: 2 January 2016 / Published: 11 January 2016
(This article belongs to the Section Chemical Sensors)
View Full-Text   |   Download PDF [3009 KB, uploaded 11 January 2016]   |  

Abstract

A fluorescence chemosensor, 2-hydroxy-1-naphthaldehyde azine (HNA) was designed and synthesized for sequential detection of Cu2+ and biothiols. It was found that HNA can specifically bind to Cu2+ with 1:1 stoichiometry, accompanied with a dramatic fluorescence quenching and a remarkable bathochromic-shift of the absorbance peak in HEPES buffer. The generated HNA-Cu2+ ensemble displayed a “turn-on” fluorescent response specific for biothiols (Hcy, Cys and GSH) based on the displacement approach, giving a remarkable recovery of fluorescence and UV-Vis spectra. The detection limits of HNA-Cu2+ to Hcy, Cys and GSH were estimated to be 1.5 μM, 1.0 μM and 0.8 μM, respectively, suggesting that HNA-Cu2+ is sensitive enough for the determination of thiols in biological systems. The biocompatibility of HNA towards A549 human lung carcinoma cell, was evaluated by an MTT assay. The capability of HNA-Cu2+ to detect biothiols in live A549 cells was then demonstrated by a microscopy fluorescence imaging assay. View Full-Text
Keywords: aldazine; chemosensing ensemble; biothiols; detection; fluorescence imaging aldazine; chemosensing ensemble; biothiols; detection; fluorescence imaging
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MDPI and ACS Style

Jia, H.; Yang, M.; Meng, Q.; He, G.; Wang, Y.; Hu, Z.; Zhang, R.; Zhang, Z. Synthesis and Application of an Aldazine-Based Fluorescence Chemosensor for the Sequential Detection of Cu2+ and Biological Thiols in Aqueous Solution and Living Cells. Sensors 2016, 16, 79.

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