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Erratum published on 25 April 2017, see Sensors 2017, 17(5), 945.

Open AccessArticle
Sensors 2015, 15(9), 22672-22691; doi:10.3390/s150922672

Novel PCR Assays Complement Laser Biosensor-Based Method and Facilitate Listeria Species Detection from Food

1
Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN 47907, USA
2
Department of Food Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonbuk 561756, Korea
3
Department of Comparative Pathobiology, Purdue University, West Lafayette, IN 47907, USA
Present address: CROUS de Dijon, Dijon Cedex 21012, France.
These authors contributed equally to this study.
*
Author to whom correspondence should be addressed.
Academic Editor: Alexander Star
Received: 4 June 2015 / Accepted: 1 September 2015 / Published: 8 September 2015
(This article belongs to the Special Issue Sensors for Food Safety and Quality)
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Abstract

The goal of this study was to develop the Listeria species-specific PCR assays based on a house-keeping gene (lmo1634) encoding alcohol acetaldehyde dehydrogenase (Aad), previously designated as Listeria adhesion protein (LAP), and compare results with a label-free light scattering sensor, BARDOT (bacterial rapid detection using optical scattering technology). PCR primer sets targeting the lap genes from the species of Listeria sensu stricto were designed and tested with 47 Listeria and 8 non-Listeria strains. The resulting PCR primer sets detected either all species of Listeria sensu stricto or individual L. innocua, L. ivanovii and L. seeligeri, L. welshimeri, and L. marthii without producing any amplified products from other bacteria tested. The PCR assays with Listeria sensu stricto-specific primers also successfully detected all species of Listeria sensu stricto and/or Listeria innocua from mixed culture-inoculated food samples, and each bacterium in food was verified by using the light scattering sensor that generated unique scatter signature for each species of Listeria tested. The PCR assays based on the house-keeping gene aad (lap) can be used for detection of either all species of Listeria sensu stricto or certain individual Listeria species in a mixture from food with a detection limit of about 104 CFU/mL. View Full-Text
Keywords: alcohol acetaldehyde dehydrogenase; Listeria adhesion protein; Listeria species; PCR; detection; BARDOT alcohol acetaldehyde dehydrogenase; Listeria adhesion protein; Listeria species; PCR; detection; BARDOT
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kim, K.-P.; Singh, A.K.; Bai, X.; Leprun, L.; Bhunia, A.K. Novel PCR Assays Complement Laser Biosensor-Based Method and Facilitate Listeria Species Detection from Food. Sensors 2015, 15, 22672-22691.

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