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Sensors 2015, 15(10), 25831-25867; doi:10.3390/s151025831

Fluorescence-Based Bioassays for the Detection and Evaluation of Food Materials

1
Department of Applied Chemistry and Biochemistry, Faculty of Engineering, Kyushu Sangyo University, 2-3-1 Matsukadai, Kasi-i, Higashi-ku, Fukuoka 813-8503, Japan
2
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan
3
Scinet Company, 4-21-12 Takanawa, Minato-ku, Tokyo 108-0074, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Arun Bhunia
Received: 24 July 2015 / Revised: 28 September 2015 / Accepted: 30 September 2015 / Published: 13 October 2015
(This article belongs to the Special Issue Sensors for Food Safety and Quality)
View Full-Text   |   Download PDF [799 KB, uploaded 13 October 2015]

Abstract

We summarize here the recent progress in fluorescence-based bioassays for the detection and evaluation of food materials by focusing on fluorescent dyes used in bioassays and applications of these assays for food safety, quality and efficacy. Fluorescent dyes have been used in various bioassays, such as biosensing, cell assay, energy transfer-based assay, probing, protein/immunological assay and microarray/biochip assay. Among the arrays used in microarray/biochip assay, fluorescence-based microarrays/biochips, such as antibody/protein microarrays, bead/suspension arrays, capillary/sensor arrays, DNA microarrays/polymerase chain reaction (PCR)-based arrays, glycan/lectin arrays, immunoassay/enzyme-linked immunosorbent assay (ELISA)-based arrays, microfluidic chips and tissue arrays, have been developed and used for the assessment of allergy/poisoning/toxicity, contamination and efficacy/mechanism, and quality control/safety. DNA microarray assays have been used widely for food safety and quality as well as searches for active components. DNA microarray-based gene expression profiling may be useful for such purposes due to its advantages in the evaluation of pathway-based intracellular signaling in response to food materials. View Full-Text
Keywords: fluorescent dye; bioassay; food study; microarray; signaling pathway fluorescent dye; bioassay; food study; microarray; signaling pathway
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Nishi, K.; Isobe, S.-I.; Zhu, Y.; Kiyama, R. Fluorescence-Based Bioassays for the Detection and Evaluation of Food Materials. Sensors 2015, 15, 25831-25867.

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