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Sensors 2015, 15(8), 20543-20556; doi:10.3390/s150820543

DNA-Redox Cation Interaction Improves the Sensitivity of an Electrochemical Immunosensor for Protein Detection

1
Department of Chemistry, Simon Fraser University, 8888 University Drive, Burnaby, BC V5A 1S6, Canada
2
Biogate Laboratories Ltd., 110-4238 Lozells Avenue, Burnaby, BC V5A 0C4, Canada
*
Author to whom correspondence should be addressed.
Academic Editor: Kagan Kerman
Received: 22 June 2015 / Revised: 31 July 2015 / Accepted: 12 August 2015 / Published: 20 August 2015
View Full-Text   |   Download PDF [657 KB, uploaded 20 August 2015]   |  

Abstract

A simple DNA-redox cation interaction enhancement strategy has been developed to improve the sensitivity of electrochemical immunosensors for protein detection. Instead of labeling with fluorophores or redox-active groups, the detection antibodies were tethered with DNA single strands. Based on the electrostatic interaction between redox cations ([Ru(NH3)6]3+) and negatively charged DNA backbone, enhanced electrochemical signals were obtained. Human chorionic gonadotropin (hCG) detection has been performed as a trial analysis. A linear response range up to the concentration of 25 mIU/mL and a detection limit of 1.25 mIU/mL have been achieved, both are comparable with the ultrasensitive enzyme-linked immunosorbent assay (ELISA) tests. The method also shows great selectivity towards hCG over other hormones such as thyroid stimulating hormone (TSH) and follicle stimulating hormone (FSH). By and large, our approach bears the merits of cost effectiveness and simplicity of instrumentation in comparison with conventional optical detection methods. View Full-Text
Keywords: electrochemical immunoassay; redox labeling; signal enhancement; DNA-cation interaction; hCG electrochemical immunoassay; redox labeling; signal enhancement; DNA-cation interaction; hCG
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Li, P.; Ge, B.; Ou, L.M.-L.; Yao, Z.; Yu, H.-Z. DNA-Redox Cation Interaction Improves the Sensitivity of an Electrochemical Immunosensor for Protein Detection. Sensors 2015, 15, 20543-20556.

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