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Sensors 2015, 15(8), 19819-19829; doi:10.3390/s150819819

Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique

1
Center for Biomedical Technology, Optics, Photonics and Biophotonics Lab, Universidad Politécnica de Madrid. Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain
2
Department of Applied Physics and Material, Escuela Técnica Superior de Ingenieros Industriales (ETSII), Universidad Politécnica de Madrid, Jose Gutiérrez Abascal, 2. 28006 Madrid, Spain
3
BioOpticalDetection, Centro de Empresas de la UPM, Campus Montegancedo, 28223 Pozuelo de Alarcón, Madrid, Spain
4
Bioftalmik. Parque Tecnológico Zamudio Ed. 800 2ª Planta 48160, Bizkaia, Spain
5
AntibodyBcn, MRB 104 Modul b UAB Campus, 08193 Bellaterra, Barcelona, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Stephen Holler
Received: 30 June 2015 / Revised: 5 August 2015 / Accepted: 6 August 2015 / Published: 13 August 2015
(This article belongs to the Special Issue Label-Free Sensing)
View Full-Text   |   Download PDF [353 KB, uploaded 25 August 2015]   |  

Abstract

The specificity and affinity of antibody-antigen interactions is a fundamental way to achieve reliable biosensing responses. Different proteins involved with dry eye dysfunction: ANXA1, ANXA11, CST4, PRDX5, PLAA and S100A6; were validated as biomarkers. In this work several antibodies were tested for ANXA1, ANXA11 and PRDX5 to select the best candidates for each biomarker. The results were obtained by using Biophotonic Sensing Cells (BICELLs) as an efficient methodology for label-free biosensing and compared with the Enzyme-Linked Immuno Sorbent Assay (ELISA) technique. View Full-Text
Keywords: photonic sensing cells BICELLs; biosensors; label-free immunoassay; dry eye biomarkers photonic sensing cells BICELLs; biosensors; label-free immunoassay; dry eye biomarkers
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Laguna, M.; Holgado, M.; Hernandez, A.L.; Santamaría, B.; Lavín, A.; Soria, J.; Suarez, T.; Bardina, C.; Jara, M.; Sanza, F.J.; Casquel, R. Antigen-Antibody Affinity for Dry Eye Biomarkers by Label Free Biosensing. Comparison with the ELISA Technique. Sensors 2015, 15, 19819-19829.

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