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Sensors 2015, 15(8), 20511-20523; doi:10.3390/s150820511

Considerations on Circuit Design and Data Acquisition of a Portable Surface Plasmon Resonance Biosensing System

1
Department of Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China
2
School of Human Nutrition and Dietetics, McGill University, Ste Anne de Bellevue, QC H9X 3V9, Canada
3
Department of Electronic and Telecommunications, University of Gävle, Gävle SE-801 76, Sweden
4
College of life sciences, Henan Agricultural University, Zhengzhou 450002, China
5
State key laboratory of wheat and maize crop science, Zhengzhou 450002, China
*
Author to whom correspondence should be addressed.
Academic Editor: Stephen Holler
Received: 15 June 2015 / Revised: 6 August 2015 / Accepted: 7 August 2015 / Published: 19 August 2015
(This article belongs to the Special Issue Label-Free Sensing)
View Full-Text   |   Download PDF [454 KB, uploaded 20 August 2015]   |  

Abstract

The aim of this study was to develop a circuit for an inexpensive portable biosensing system based on surface plasmon resonance spectroscopy. This portable biosensing system designed for field use is characterized by a special structure which consists of a microfluidic cell incorporating a right angle prism functionalized with a biomolecular identification membrane, a laser line generator and a data acquisition circuit board. The data structure, data memory capacity and a line charge-coupled device (CCD) array with a driving circuit for collecting the photoelectric signals are intensively focused on and the high performance analog-to-digital (A/D) converter is comprehensively evaluated. The interface circuit and the photoelectric signal amplifier circuit are first studied to obtain the weak signals from the line CCD array in this experiment. Quantitative measurements for validating the sensitivity of the biosensing system were implemented using ethanol solutions of various concentrations indicated by volume fractions of 5%, 8%, 15%, 20%, 25%, and 30%, respectively, without a biomembrane immobilized on the surface of the SPR sensor. The experiments demonstrated that it is possible to detect a change in the refractive index of an ethanol solution with a sensitivity of 4.99838 × 105 ΔRU/RI in terms of the changes in delta response unit with refractive index using this SPR biosensing system, whereby the theoretical limit of detection of 3.3537 × 10−5 refractive index unit (RIU) and a high linearity at the correlation coefficient of 0.98065. The results obtained from a series of tests confirmed the practicality of this cost-effective portable SPR biosensing system. View Full-Text
Keywords: surface plasmon resonance; biosensing system; linear CCD array; microcontroller; ADC conversion time; refractive index surface plasmon resonance; biosensing system; linear CCD array; microcontroller; ADC conversion time; refractive index
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Chang, K.; Chen, R.; Wang, S.; Li, J.; Hu, X.; Liang, H.; Cao, B.; Sun, X.; Ma, L.; Zhu, J.; Jiang, M.; Hu, J. Considerations on Circuit Design and Data Acquisition of a Portable Surface Plasmon Resonance Biosensing System. Sensors 2015, 15, 20511-20523.

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