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Sensors 2015, 15(4), 7605-7618; doi:10.3390/s150407605

An Improved Electrochemical Aptasensor for Chloramphenicol Detection Based on Aptamer Incorporated Gelatine

1
AXES Research Group, Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium
2
Sphere Research Group, Department of Biology, University of Antwerp, Groenenborgerlaan 171, B-2020 Antwerp, Belgium
*
Author to whom correspondence should be addressed.
Academic Editor: Kagan Kerman
Received: 13 January 2015 / Revised: 9 March 2015 / Accepted: 16 March 2015 / Published: 27 March 2015
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Abstract

Because of the biocompatible properties of gelatine and the good affinity of aptamers for their targets, the combination of aptamer and gelatine type B is reported as promising for the development of biosensing devices. Here, an aptamer for chloramphenicol (CAP) is mixed with different types of gelatine and dropped on the surface of disposable gold screen printed electrodes. The signal of the CAP reduction is investigated using differential pulse voltammetry. The diagnostic performance of the sensor is described and a detection limit of 1.83 × 10−10 M is found. The selectivity and the stability of the aptasensor are studied and compared to those of other CAP sensors described in literature. View Full-Text
Keywords: aptamers; gelatine; chloramphenicol; electrochemical sensor aptamers; gelatine; chloramphenicol; electrochemical sensor
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Hamidi-Asl, E.; Dardenne, F.; Blust, R.; De Wael, K. An Improved Electrochemical Aptasensor for Chloramphenicol Detection Based on Aptamer Incorporated Gelatine. Sensors 2015, 15, 7605-7618.

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