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Sensors 2015, 15(2), 4353-4367; doi:10.3390/s150204353

A Choline Oxidase Amperometric Bioassay for the Detection of Mustard Agents Based on Screen-Printed Electrodes Modified with Prussian Blue Nanoparticles

1
Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, Via Della Ricerca Scientifica, 00133 Rome, Italy
2
Dipartimento Agroalimentare, IC-CNR Istituto di Cristallografia, Via Salaria Km 29.3, 00015 Monterotondo Scalo, Rome 00015, Italy
3
Laboratoire Génie des Procédés et Environnement, Faculté de Sciences et Techniques, Université Hassan II—Mohammedia, B.P.146, Mohammadia 20850, Morocco
*
Author to whom correspondence should be addressed.
Received: 31 December 2014 / Revised: 4 February 2015 / Accepted: 6 February 2015 / Published: 13 February 2015
(This article belongs to the Section Chemical Sensors)
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Abstract

In this work a novel bioassay for mustard agent detection was proposed. The bioassay is based on the capability of these compounds to inhibit the enzyme choline oxidase. The enzymatic activity, which is correlated to the mustard agents, was electrochemically monitored measuring the enzymatic product, hydrogen peroxide, by means of a screen-printed electrode modified with Prussian Blue nanoparticles. Prussian Blue nanoparticles are able to electrocatalyse the hydrogen peroxide concentration reduction at low applied potential (−50 mV vs. Ag/AgCl), thus allowing the detection of the mustard agents with no electrochemical interferences. The suitability of this novel bioassay was tested with the nitrogen mustard simulant bis(2-chloroethyl)amine and the sulfur mustard simulants 2-chloroethyl ethyl sulfide and 2-chloroethyl phenyl sulfide. The bioassay proposed in this work allowed the detection of mustard agent simulants with good sensitivity and fast response, which are excellent premises for the development of a miniaturised sensor well suited for an alarm system in case of terrorist attacks. View Full-Text
Keywords: mustard agents; inhibition; choline oxidase; screen-printed electrode; Prussian Blue nanoparticles mustard agents; inhibition; choline oxidase; screen-printed electrode; Prussian Blue nanoparticles
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Arduini, F.; Scognamiglio, V.; Covaia, C.; Amine, A.; Moscone, D.; Palleschi, G. A Choline Oxidase Amperometric Bioassay for the Detection of Mustard Agents Based on Screen-Printed Electrodes Modified with Prussian Blue Nanoparticles. Sensors 2015, 15, 4353-4367.

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