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Sensors 2015, 15(2), 2438-2452; doi:10.3390/s150202438

A 3D Microfluidic Chip for Electrochemical Detection of Hydrolysed Nucleic Bases by a Modified Glassy Carbon Electrode

Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, Brno CZ-613 00, Czech Republic
Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, Brno CZ-616 00, Czech Republic
Faculty of Chemistry, University of Wroclaw, 14 Joliot-Curie, Wroclaw PL-50383, Poland
Karel Englis College, Sujanovo nam. 356/1, Brno CZ-602 00, Czech Republic
Author to whom correspondence should be addressed.
Received: 22 October 2014 / Revised: 1 December 2014 / Accepted: 4 January 2015 / Published: 22 January 2015
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Modification of carbon materials, especially graphene-based materials, has wide applications in electrochemical detection such as electrochemical lab-on-chip devices. A glassy carbon electrode (GCE) modified with chemically alternated graphene oxide was used as a working electrode (glassy carbon modified by graphene oxide with sulphur containing compounds and Nafion) for detection of nucleobases in hydrolysed samples (HCl pH = 2.9, 100 °C, 1 h, neutralization by NaOH). It was found out that modification, especially with trithiocyanuric acid, increased the sensitivity of detection in comparison with pure GCE. All processes were finally implemented in a microfluidic chip formed with a 3D printer by fused deposition modelling technology. As a material for chip fabrication, acrylonitrile butadiene styrene was chosen because of its mechanical and chemical stability. The chip contained the one chamber for the hydrolysis of the nucleic acid and another for the electrochemical detection by the modified GCE. This chamber was fabricated to allow for replacement of the GCE. View Full-Text
Keywords: biosensor; lab-on-chip; electrochemistry; graphene; modification; hydrolysis; trithiocyanuric acid biosensor; lab-on-chip; electrochemistry; graphene; modification; hydrolysis; trithiocyanuric acid

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Vlachova, J.; Tmejova, K.; Kopel, P.; Korabik, M.; Zitka, J.; Hynek, D.; Kynicky, J.; Adam, V.; Kizek, R. A 3D Microfluidic Chip for Electrochemical Detection of Hydrolysed Nucleic Bases by a Modified Glassy Carbon Electrode. Sensors 2015, 15, 2438-2452.

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