Sensors 2015, 15(1), 1945-1953; doi:10.3390/s150101945
Fluorescent Cellular Assay for Screening Agents Inhibiting Pseudomonas aeruginosa Adherence
1
Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 8, 128 40 Prague 2, Czech Republic
2
Central European Institute of Technology, Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic
*
Author to whom correspondence should be addressed.
Received: 27 October 2014 / Accepted: 9 January 2015 / Published: 16 January 2015
(This article belongs to the Special Issue Selected Papers from XIV. Workshop of Physical Chemists and Electrochemists)
Abstract
Antibodies against Pseudomonas aeruginosa (PA) lectin, PAIIL, which is a virulence factor mediating the bacteria binding to epithelium cells, were prepared in chickens and purified from egg yolks. To examine these antibodies as a prophylactic agent preventing the adhesion of PA we developed a well plate assay based on fluorescently labeled bacteria and immortalized epithelium cell lines derived from normal and cystic fibrosis (CF) human lungs. The antibodies significantly inhibited bacteria adhesion (up to 50%) in both cell lines. In agreement with in vivo data, our plate assay showed higher susceptibility of CF cells towards the PA adhesion as compared to normal epithelium. This finding proved the reliability of the developed experimental system. View Full-TextKeywords:
cystic fibrosis; lungs; bacterial infection; lectin; chicken antibody; cell line; dual fluorescence
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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MDPI and ACS Style
Nosková, L.; Kubíčková, B.; Vašková, L.; Bláhová, B.; Wimmerová, M.; Stiborová, M.; Hodek, P. Fluorescent Cellular Assay for Screening Agents Inhibiting Pseudomonas aeruginosa Adherence. Sensors 2015, 15, 1945-1953.