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Sensors 2015, 15(10), 25716-25729; doi:10.3390/s151025716

A Spectrometric Method for Hydrogen Peroxide Concentration Measurement with a Reusable and Cost-Efficient Sensor

Department of Photonics Engineering, Yuan Ze University, 135 Yuan-Tung Road, Chung-Li 32003, Taiwan
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Academic Editor: W. Rudolf Seitz
Received: 7 August 2015 / Revised: 28 September 2015 / Accepted: 29 September 2015 / Published: 12 October 2015
(This article belongs to the Section Chemical Sensors)
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Abstract

In this study we developed a low cost sensor for measuring the concentration of hydrogen peroxide (H2O2) in liquids utilizing a spectrometric method. The sensor was tested using various concentrations of a peroxidase enzyme immobilized on a glass substrate. H2O2 can be catalyzed by peroxidase and converted into water and oxygen. The reagent 4-amino-phenazone takes up oxygen together with phenol to form a colored product that has absorption peaks at 510 nm and 450 nm. The transmission intensity is strongly related to the hydrogen peroxide concentration, so can be used for quantitative analysis. The measurement range for hydrogen peroxide is from 5 × 105% to 1 × 10−3% (0.5 ppm to 10 ppm) and the results show high linearity. This device can achieve a sensitivity and resolution of 41,400 (photon count/%) and 3.49 × 10−5% (0.35 ppm), respectively. The response time of the sensor is less than 3 min and the sensor can be reused for 10 applications with similar performance. View Full-Text
Keywords: optical sensor; spectrometry; hydrogen peroxide optical sensor; spectrometry; hydrogen peroxide
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Hsu, C.-C.; Lo, Y.-R.; Lin, Y.-C.; Shi, Y.-C.; Li, P.-L. A Spectrometric Method for Hydrogen Peroxide Concentration Measurement with a Reusable and Cost-Efficient Sensor. Sensors 2015, 15, 25716-25729.

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