Next Article in Journal
Carbon Fiber Ultramicrodic Electrode Electrodeposited with Over-Oxidized Polypyrrole for Amperometric Detection of Vesicular Exocytosis from Pheochromocytoma Cell
Next Article in Special Issue
Determination of Zinc, Cadmium and Lead Bioavailability in Contaminated Soils at the Single-Cell Level by a Combination of Whole-Cell Biosensors and Flow Cytometry
Previous Article in Journal
A Multi-Channel, Flex-Rigid ECoG Microelectrode Array for Visual Cortical Interfacing
Previous Article in Special Issue
Engineering Genetically Encoded FRET Sensors
Article Menu

Export Article

Open AccessArticle
Sensors 2015, 15(1), 855-867; doi:10.3390/s150100855

Hyper, a Hydrogen Peroxide Sensor, Indicates the Sensitivity of the Arabidopsis Root Elongation Zone to Aluminum Treatment

Department of Plant Molecular Biology, Institute of Biotechnology, National Autonomous University of Mexico, 62210 Mexico
Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, USA
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 4 July 2014 / Accepted: 3 December 2014 / Published: 6 January 2015
(This article belongs to the Special Issue Intracellular Sensing)
View Full-Text   |   Download PDF [1979 KB, uploaded 6 January 2015]   |  


Emerging evidence indicates that some reactive oxygen species (ROS), such as the superoxide anion radical and hydrogen peroxide (H2O2), are central regulators of plant responses to biotic and abiotic stresses. Thus, the cellular levels of ROS are thought to be tightly regulated by an efficient and elaborate pro- and antioxidant system that modulates the production and scavenging of ROS. Until recently, studies of ROS in plant cells have been limited to biochemical assays and the use of fluorescent probes; however, the irreversible oxidation of these fluorescent probes makes it impossible to visualize dynamic changes in ROS levels. In this work, we describe the use of Hyper, a recently developed live cell probe for H2O2 measurements in living cells, to monitor oxidative stress in Arabidopsis roots subjected to aluminum treatment. Hyper consists of a circularly permuted YFP (cpYFP) inserted into the regulatory domain of the Escherichia coli hydrogen peroxide-binding protein (OxyR), and is a H2O2-specific ratiometric, and therefore quantitative, probe that can be expressed in plant and animal cells. Now we demonstrate that H2O2 levels drop sharply in the elongation zone of roots treated with aluminum. This response could contribute to root growth arrest and provides evidence that H2O2 is involved in early Al sensing. View Full-Text
Keywords: Hyper; reactive oxygen species; aluminum; polar growth; Arabidopsis; plant root Hyper; reactive oxygen species; aluminum; polar growth; Arabidopsis; plant root

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Hernández-Barrera, A.; Velarde-Buendía, A.; Zepeda, I.; Sanchez, F.; Quinto, C.; Sánchez-Lopez, R.; Cheung, A.Y.; Wu, H.-M.; Cardenas, L. Hyper, a Hydrogen Peroxide Sensor, Indicates the Sensitivity of the Arabidopsis Root Elongation Zone to Aluminum Treatment. Sensors 2015, 15, 855-867.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top