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Sensors 2015, 15(1), 855-867; doi:10.3390/s150100855

Hyper, a Hydrogen Peroxide Sensor, Indicates the Sensitivity of the Arabidopsis Root Elongation Zone to Aluminum Treatment

1
Department of Plant Molecular Biology, Institute of Biotechnology, National Autonomous University of Mexico, 62210 Mexico
2
Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, USA
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 4 July 2014 / Accepted: 3 December 2014 / Published: 6 January 2015
(This article belongs to the Special Issue Intracellular Sensing)
View Full-Text   |   Download PDF [1979 KB, uploaded 6 January 2015]   |  

Abstract

Emerging evidence indicates that some reactive oxygen species (ROS), such as the superoxide anion radical and hydrogen peroxide (H2O2), are central regulators of plant responses to biotic and abiotic stresses. Thus, the cellular levels of ROS are thought to be tightly regulated by an efficient and elaborate pro- and antioxidant system that modulates the production and scavenging of ROS. Until recently, studies of ROS in plant cells have been limited to biochemical assays and the use of fluorescent probes; however, the irreversible oxidation of these fluorescent probes makes it impossible to visualize dynamic changes in ROS levels. In this work, we describe the use of Hyper, a recently developed live cell probe for H2O2 measurements in living cells, to monitor oxidative stress in Arabidopsis roots subjected to aluminum treatment. Hyper consists of a circularly permuted YFP (cpYFP) inserted into the regulatory domain of the Escherichia coli hydrogen peroxide-binding protein (OxyR), and is a H2O2-specific ratiometric, and therefore quantitative, probe that can be expressed in plant and animal cells. Now we demonstrate that H2O2 levels drop sharply in the elongation zone of roots treated with aluminum. This response could contribute to root growth arrest and provides evidence that H2O2 is involved in early Al sensing. View Full-Text
Keywords: Hyper; reactive oxygen species; aluminum; polar growth; Arabidopsis; plant root Hyper; reactive oxygen species; aluminum; polar growth; Arabidopsis; plant root
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Hernández-Barrera, A.; Velarde-Buendía, A.; Zepeda, I.; Sanchez, F.; Quinto, C.; Sánchez-Lopez, R.; Cheung, A.Y.; Wu, H.-M.; Cardenas, L. Hyper, a Hydrogen Peroxide Sensor, Indicates the Sensitivity of the Arabidopsis Root Elongation Zone to Aluminum Treatment. Sensors 2015, 15, 855-867.

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