Next Article in Journal
An Adaptive Jitter Mechanism for Reactive Route Discovery in Sensor Networks
Previous Article in Journal
A Unitary ESPRIT Scheme of Joint Angle Estimation for MOTS MIMO Radar
Article Menu

Export Article

Open AccessArticle
Sensors 2014, 14(8), 14423-14439; doi:10.3390/s140814423

Electrochemical Quantification of the Antioxidant Capacity of Medicinal Plants Using Biosensors

1
Departamento de Química, Universidad Autónoma Metropolitana-Iztapalapa, Área de Química Analítica, San Rafael Atlixco 186, Col. Vicentina, Del. Iztapalapa, México D.F., C.P. 09340, Mexico
2
Departamento de Materiales, Universidad Autónoma Metropolitana-Azcapotzalco, Área Ingeniería de Materiales, Av. San Pablo 180, Col. Reynosa-Tamaulipas, Del. Azcapotzalco, México, D.F., C.P. 02200, Mexico
3
SEP-Instituto Tecnológico de Toluca. Departamento de Ingeniería Química y Bioquímica. Av, Tecnológico S/N. Fraccionamiento La Virgen, Metepec, Edo de México, C.P. 52149, Mexico
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 30 June 2014 / Revised: 28 July 2014 / Accepted: 30 July 2014 / Published: 8 August 2014
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [767 KB, uploaded 11 August 2014]   |  

Abstract

The working area of a screen-printed electrode, SPE, was modified with the enzyme tyrosinase (Tyr) using different immobilization methods, namely entrapment with water-soluble polyvinyl alcohol (PVA), cross-linking using glutaraldehyde (GA), and cross-linking using GA and human serum albumin (HSA); the resulting electrodes were termed SPE/Tyr/PVA, SPE/Tyr/GA and SPE/Tyr/HSA/GA, respectively. These biosensors were characterized by means of amperometry and EIS techniques. From amperometric evaluations, the apparent Michaelis-Menten constant, Km′, of each biosensor was evaluated while the respective charge transfer resistance, Rct, was assessed from impedance measurements. It was found that the SPE/Tyr/GA had the smallest Km′ (57 ± 7) µM and Rct values. This electrode also displayed both the lowest detection and quantification limits for catechol quantification. Using the SPE/Tyr/GA, the Trolox Equivalent Antioxidant Capacity (TEAC) was determined from infusions prepared with “mirto” (Salvia microphylla), “hHierba dulce” (Lippia dulcis) and “salve real” (Lippia alba), medicinal plants commonly used in Mexico. View Full-Text
Keywords: medicinal plants; antioxidant capacity; biosensors; tyrosinase; immobilization; screen-printed electrodes; Michaelis-Menten constant medicinal plants; antioxidant capacity; biosensors; tyrosinase; immobilization; screen-printed electrodes; Michaelis-Menten constant
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Rodríguez-Sevilla, E.; Ramírez-Silva, M.-T.; Romero-Romo, M.; Ibarra-Escutia, P.; Palomar-Pardavé, M. Electrochemical Quantification of the Antioxidant Capacity of Medicinal Plants Using Biosensors. Sensors 2014, 14, 14423-14439.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top